Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
Comp Biochem Physiol B Biochem Mol Biol. 2011 Apr;158(4):282-8. doi: 10.1016/j.cbpb.2010.12.005. Epub 2011 Jan 1.
l-Gulono-gamma-lactone oxidase (GULO) is a key enzyme for the biosynthesis of ascorbate, which is essential for several cellular functions. In the present study, mRNA expression of GULO gene was evaluated during the early development of Persian sturgeon. First, because there are no comparative studies that have established suitable quantitative real-time PCR reference genes in sturgeons for any physiological conditions, we evaluated six candidate reference genes (ACTB, RPL13, UBQ, RPL6, GAPDH and EF1A) during the early development of Persian sturgeon. The most stable mRNA expression was obtained with RPL6 and ACTB, whereas the least stable was RPL13. After normalization using RPL6, ACTB and RPL6/ACTB combination, the mRNA expression of GULO was highest at the embryonic stage (2days before hatching; P<0.05) and started to decline from hatching of larvae to the rest of the developmental time-points. This suggests that the vitamin C requirements are highest during early life stages, and it is likely that the changes in GULO mRNA expression are associated with changes in GULO enzyme activity.
L-古洛糖酸-γ-内酯氧化酶（GULO）是抗坏血酸生物合成的关键酶，抗坏血酸对许多细胞功能至关重要。在本研究中，评估了波斯鲟早期发育过程中 GULO 基因的 mRNA 表达。首先，由于在任何生理条件下，鲟鱼中都没有建立合适的定量实时 PCR 内参基因的比较研究，因此我们在波斯鲟早期发育过程中评估了 6 个候选内参基因（ACTB、RPL13、UBQ、RPL6、GAPDH 和 EF1A）。最稳定的 mRNA 表达是使用 RPL6 和 ACTB 获得的，而最不稳定的是 RPL13。使用 RPL6、ACTB 和 RPL6/ACTB 组合归一化后，GULO 的 mRNA 表达在胚胎期（孵化前 2 天；P<0.05）最高，并且从孵化开始到发育的其余时间点开始下降。这表明在生命早期阶段对维生素 C 的需求最高，并且 GULO mRNA 表达的变化可能与 GULO 酶活性的变化有关。
