Abdolahnejad Zeinab, Pourkazemi Mohammad, Khoshkholgh Majid Reza, Yarmohammadi Mahtab
Fisheries Department, Faculty of Natural Resources, University of Guilan Sowmeh Sara, Iran.
Iranian Fisheries Science Research Institute/Agriculture Research Education Extension, Organization, Tehran, Iran.
Mol Biol Res Commun. 2015 Dec;4(4):181-188.
The mRNA expression of growth hormone () gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of mRNA expression. The results showed that the mRNA could be observed in the eyed eggs and even at unfertilized eggs of Siberian sturgeon. The highest amounts of mRNA were found at 25 and 50 dph larvae, while the lowest levels were detected at 1 and 3 dph larvae stage. These findings suggest that, the mRNA play a key role during developmental stages of Siberian sturgeon.
采用RT-PCR方法研究了西伯利亚鲟早期发育阶段生长激素（）基因的mRNA表达。在11个不同时间点收集了从未受精卵到孵化后50天（dph）幼虫的样本。在定量mRNA表达过程中，核糖体蛋白L6（RPL6）转录本用作内标。结果表明，在西伯利亚鲟的有眼卵甚至未受精卵中都能观察到mRNA。在25和50 dph幼虫中发现mRNA含量最高，而在1和3 dph幼虫阶段检测到的水平最低。这些发现表明，mRNA在西伯利亚鲟的发育阶段起着关键作用。
