Tozuka S, Uchida T, Suzuki K, Esumi M, Shikata T
Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
Arch Pathol Lab Med. 1989 Jan;113(1):20-5.
By Southern blot hybridization, the state of hepatitis B virus (HBV) in the liver was investigated by utilizing needle biopsy specimens of 59 patients with hepatitis B surface antigen-positive noncarcinomatous liver disease. The tissue HBV DNA revealed a replicative form in 23 patients with chronic active hepatitis (48%) and an integrated form in 23 (48%) of these 48 patients. A liver with a replicative form was more frequently associated with hepatitis Be antigen (HBeAg), serum HBV DNA, and expression of hepatitis B core antigen than one without it. Integration of HBV DNA was more common in the anti-hepatitis Be phase or in the absence of necroinflammatory activity, and its frequency of occurrence roughly paralleled progression of liver disease: early, 21%; advanced, 56%; and cirrhotic stage, 61%.
通过Southern印迹杂交法，利用59例乙型肝炎表面抗原阳性的非癌性肝病患者的肝穿刺活检标本，对肝脏中乙型肝炎病毒（HBV）的状态进行了研究。组织HBV DNA在23例慢性活动性肝炎患者（48%）中显示为复制型，在这48例患者中的23例（48%）中显示为整合型。具有复制型的肝脏比没有复制型的肝脏更常与乙型肝炎e抗原（HBeAg）、血清HBV DNA以及乙型肝炎核心抗原的表达相关。HBV DNA整合在抗乙型肝炎e期或无坏死性炎症活动时更为常见，其发生频率大致与肝病进展平行：早期为21%；晚期为56%；肝硬化阶段为61%。
