在铜绿假单胞菌IFO 3080及其他菌株中，D-鼠李聚糖作为与单克隆抗体E87发生反应的共同抗原的出现情况。
Occurrence of D-rhamnan as the common antigen reactive against monoclonal antibody E87 in Pseudomonas aeruginosa IFO 3080 and other strains.
作者信息
Yokota S, Kaya S, Araki Y, Ito E, Kawamura T, Sawada S
机构信息
Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan.
出版信息
J Bacteriol. 1990 Oct;172(10):6162-4. doi: 10.1128/jb.172.10.6162-6164.1990.
S. Sawada and co-workers reported that a monoclonal antibody (MAb), E87, interacted with about 80% of Pseudomonas aeruginosa isolates, and they separated a rhamnose-rich polysaccharide as the probable antigen for MAb E87 from P. aeruginosa IFO 3080 (S. Sawada, T. Kawamura, Y. Masuho, and K. Tomibe, J. Infec. Dis. 152:1290-1299, 1985). In the present study, the rhamnose-rich polysaccharide was shown to be structurally and immunologically identical to the D-rhamnan of P. aeruginosa IID 1008 (S. Yokota, S. Kaya, S. Sawada, T. Kawamura, Y. Araki, and E. Ito, Eur. J. Biochem. 167:203-209, 1987). Furthermore, a set of enzymes responsible for the formation of GDP-rhamnose (probably in a D-form) from GDP-D-mannose was found in the 100,000 x g supernatant fractions obtained from all of nine P. aeruginosa strains reactive against MAb E87. The result strongly supports a possibility that lipopolysaccharides having a D-rhamnan chain widely occur as the common antigen among various P. aeruginosa isolates.
泽田等人报道，一种单克隆抗体（MAb）E87可与约80%的铜绿假单胞菌分离株发生相互作用，他们从铜绿假单胞菌IFO 3080中分离出一种富含鼠李糖的多糖，作为MAb E87的可能抗原（泽田幸司、川村彻、增穗洋、富部克己，《传染病杂志》152:1290 - 1299，1985年）。在本研究中，已证明这种富含鼠李糖的多糖在结构和免疫方面与铜绿假单胞菌IID 1008的D - 鼠李聚糖相同（横田秀树、加弥聪、泽田幸司、川村彻、荒木洋、伊藤悦郎，《欧洲生物化学杂志》167:203 - 209，1987年）。此外，在从对MAb E87有反应的所有9株铜绿假单胞菌菌株获得的100,000×g上清液组分中，发现了一组负责从GDP - D - 甘露糖形成GDP - 鼠李糖（可能是D型）的酶。该结果有力地支持了一种可能性，即具有D - 鼠李聚糖链的脂多糖作为常见抗原广泛存在于各种铜绿假单胞菌分离株中。
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