Lam M Y, McGroarty E J, Kropinski A M, MacDonald L A, Pedersen S S, Høiby N, Lam J S
Department of Microbiology, University of Guelph, Ontario, Canada.
J Clin Microbiol. 1989 May;27(5):962-7. doi: 10.1128/jcm.27.5.962-967.1989.
The lipopolysaccharide (LPS) of Pseudomonas aeruginosa PAO1 contains two species of O polysaccharide termed A and B bands. The high-molecular-weight B-band LPS determines the O specificity of the bacterium, while the antigenically distinct A-band LPS consists of only shorter-chain polysaccharides. Seven hybridomas secreting A-band-specific monoclonal antibodies were produced and used to study the LPS of standard and clinical strains. Although A-band antibodies did not agglutinate any of the serotype strains presently in the International Antigenic Typing Scheme, Western immunoblots revealed that 11 of the 17 serotype strains possessed A-band LPS. In a group of 250 clinical isolates from patients with cystic fibrosis, 170 (68%) had A-band LPS on the basis of agglutination tests, but in silver-stained gels all were shown to be deficient in O-antigen-containing B band. Investigation of serial isolates from a single patient revealed a pattern of antigenic variation. During the course of the infection, serotypeable isolates became nontypeable, and the O antigen was replaced with A band as the major LPS antigen. These results suggest that A-band LPS may be the major LPS antigen in nontypeable clinical isolates and a common antigen among other P. aeruginosa strains.
铜绿假单胞菌PAO1的脂多糖（LPS）包含两种O多糖，称为A带和B带。高分子量的B带LPS决定了该细菌的O特异性，而抗原性不同的A带LPS仅由较短链的多糖组成。制备了七种分泌A带特异性单克隆抗体的杂交瘤，并用于研究标准菌株和临床菌株的LPS。尽管A带抗体不能凝集目前国际抗原分型方案中的任何血清型菌株，但Western免疫印迹显示，17种血清型菌株中有11种具有A带LPS。在一组来自囊性纤维化患者的250份临床分离株中，基于凝集试验，170株（68%）具有A带LPS，但在银染凝胶中，所有分离株均显示缺乏含O抗原的B带。对一名患者的系列分离株进行研究发现了抗原变异模式。在感染过程中，可分型的分离株变为不可分型，并且O抗原被A带取代，成为主要的LPS抗原。这些结果表明，A带LPS可能是不可分型临床分离株中的主要LPS抗原，也是其他铜绿假单胞菌菌株中的共同抗原。
