Andrade Z A, Grimaud J A
Am J Pathol. 1986 Jul;124(1):59-65.
Mice with 64-day-old Schistosoma mansoni infection (+/- 27 worms, 8-12 pairs) were treated simultaneously with oxamniquine and hycanthone. The cure rate was 100%, and changes occurring thereafter in the liver were sequentially followed by means of histologic, ultrastructural, and immunofluorescence methods. Soon after treatment, hepatitic changes cleared up and periovular granulomas diminished in size. The predominant Type III collagen in granulomas was reduced, and the Type I showed no apparent increase, whereas Type IV did not seem to participate in the process. Collagen fibrils in periovular granulomas changed in texture from dense and more oriented to loose and disorganized. Fibroblasts, at first with marked signs of hyperfunction, became less so at a time when collagen fragments appeared within secondary lysosomes in macrophages and fibroblasts. Schistosomal ovular antigens remained sequestered inside the fibrotic granulomas up to the final, 39th day after treatment. Thus, specific treatment of schistosomiasis showed a beneficial effect upon the hepatic lesions from the very beginning and promoted changes in the periovular granulomas that indicated a rapid, although incomplete, resorption of fibrosis.
感染曼氏血吸虫64天的小鼠（±27条虫，8 - 12对）同时接受奥沙尼喹和海恩酮治疗。治愈率为100%，此后通过组织学、超微结构和免疫荧光方法依次跟踪肝脏发生的变化。治疗后不久，肝炎性改变消退，虫卵周围肉芽肿体积减小。肉芽肿中主要的III型胶原减少，I型胶原未见明显增加，而IV型胶原似乎未参与该过程。虫卵周围肉芽肿中的胶原纤维质地从致密且更具方向性变为疏松且无序。成纤维细胞最初有明显的功能亢进迹象，在巨噬细胞和成纤维细胞的次级溶酶体内出现胶原片段时，这种迹象有所减轻。直至治疗后第39天即最后阶段，血吸虫卵抗原仍被隔离在纤维化肉芽肿内。因此，血吸虫病的特异性治疗从一开始就对肝脏病变显示出有益效果，并促使虫卵周围肉芽肿发生变化，表明纤维化虽未完全但迅速吸收。
