Kaljunen Heidi, Panneerselvam Saravanan, Mueller-Dieckmann Jochen
EMBL Hamburg Outstation, Germany.
Photon Science, DESY, Germany.
Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):959-62. doi: 10.1107/S2053230X14011844. Epub 2014 Jun 19.
Enhanced disease resistance 1 is a member of the Raf-like mitogen-activated protein kinase kinase kinase (MAPKKK) family that negatively regulates disease resistance, ethylene-induced senescence and programmed cell death in response to both abiotic and biotic stresses. A catalytically inactive form of the EDR1 kinase domain was successfully cloned, expressed, purified and crystallized. Crystallization was conducted in the presence of the ATP analogue AMP-PNP. The crystals belonged to space group P3221 and contained two molecules in the asymmetric unit. The crystals diffracted X-rays to 2.55 Å resolution.
增强抗病性1是Raf样丝裂原活化蛋白激酶激酶激酶（MAPKKK）家族的成员，在响应非生物和生物胁迫时，对抗病性、乙烯诱导的衰老和程序性细胞死亡起负调控作用。成功克隆、表达、纯化并结晶了EDR1激酶结构域的催化失活形式。在ATP类似物AMP-PNP存在的情况下进行结晶。晶体属于P3221空间群，不对称单元中包含两个分子。这些晶体的X射线衍射分辨率达到2.55 Å。
