Matsunaga T, Chen T, Törmänen V
Unit for Applied Cell and Molecular Biology, University of Umea, Sweden.
Proc Natl Acad Sci U S A. 1990 Oct;87(19):7767-71. doi: 10.1073/pnas.87.19.7767.
A germ-line heavy-chain variable region (VH) gene (RTVH431) has been isolated from a rainbow trout (Salmo gairdneri) and characterized by complete nucleotide sequencing. It is characteristic of VH, as shown by the conserved octamer and TATA motif in the 5' region, the heptamernonamer recombination signal sequence in the 3' region, and the 18-amino-acid-long hydrophobic leader interrupted by an intron. The 98-amino-acid-long VH coding region has 50-70% nucleotide sequence homology and 40-60% amino acid sequence homology with VHS of various vertebrate species. We have also found unique or species-specific amino acid residues in the VHS of rainbow trout, amphibia (Xenopus), reptile (Caiman), and shark (Heterodontus) in our sequence analyses. The RTVH431 has an unusual amino acid in the conserved 34th position in complementarity-determining region 1 of VH. Southern hybridization results suggest the presence of a large gene family related to RTVH431 in the trout genome. The complex evolution of antibody V genes is discussed.
从虹鳟（Salmo gairdneri）中分离出一个种系重链可变区（VH）基因（RTVH431），并通过完整的核苷酸测序对其进行了表征。它具有VH的特征，如5'区域中保守的八聚体和TATA基序、3'区域中的七聚体-九聚体重组信号序列以及被内含子打断的18个氨基酸长的疏水前导序列。这个98个氨基酸长的VH编码区与各种脊椎动物物种的VH具有50-70%的核苷酸序列同源性和40-60%的氨基酸序列同源性。在我们的序列分析中，我们还在虹鳟、两栖动物（非洲爪蟾）、爬行动物（凯门鳄）和鲨鱼（异齿鲨）的VH中发现了独特的或物种特异性的氨基酸残基。RTVH431在VH互补决定区1保守的第34位有一个不寻常的氨基酸。Southern杂交结果表明，在鳟鱼基因组中存在一个与RTVH431相关的大基因家族。文中讨论了抗体V基因的复杂进化。
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