Ollo R, Sikorav J L, Rougeon F
Nucleic Acids Res. 1983 Nov 25;11(22):7887-97. doi: 10.1093/nar/11.22.7887.
The mouse VHIII subgroup is composed of four families which share sequence homology. We isolated a VH germ-line genomic clone, which cross hybridizes with a cDNA probe from one of these families, derived from a myeloma secreting an antigalactan antibody. We report here the nucleotide sequence of the cross hybridizing gene and show that very likely it has an anti-sheep red blood cell specificity. Comparison of its nucleotide sequence with those of the three other VHIII families shows that these genes share segmental homologies of variable lengths. This suggests that interchanges of sequence blocks between VH genes could be an important evolutionary mechanism for diversifying the germ-line repertoire. The strong homology (82%) with human VHIII genes suggests that efficient antibody sequences are strongly conserved. This conservation of homology is particularly striking when compared to the more limited homology (63%) between mouse and human C kappa genes.
小鼠VHIII亚组由四个具有序列同源性的家族组成。我们分离出一个VH种系基因组克隆，它与来自其中一个家族的cDNA探针交叉杂交，该家族源自分泌抗半乳聚糖抗体的骨髓瘤。我们在此报告交叉杂交基因的核苷酸序列，并表明它很可能具有抗绵羊红细胞特异性。将其核苷酸序列与其他三个VHIII家族的序列进行比较，结果表明这些基因具有不同长度的片段同源性。这表明VH基因之间序列块的交换可能是种系库多样化的重要进化机制。与人类VHIII基因的高度同源性（82%）表明，有效的抗体序列得到了强烈的保守。与小鼠和人类Cκ基因之间更有限的同源性（63%）相比，这种同源性的保守性尤为显著。
