Isaac Giorgis
Bio Separation and Mass Spectrometry, Pacific Northwest National Laboratory, Richland, WA, USA.
Methods Mol Biol. 2011;708:259-75. doi: 10.1007/978-1-61737-985-7_16.
In the past decade, many new strategies for mass spectrometry (MS)-based analyses of lipids have been developed. Lipidomics is one of the most promising research fields to emerge as a result of these advances in MS. Currently, mass spectrometric analysis of lipids involves two complementary approaches: direct infusion (shotgun lipidomics) and liquid chromatography coupled to MS. In this chapter, I will demonstrate the approach of shotgun lipidomics using electrospray ionization tandem MS for the analysis of lipid molecular species directly from crude biological extracts of tissue or fluids.
在过去十年中，基于质谱（MS）的脂质分析已开发出许多新策略。脂质组学是由于质谱技术的这些进展而出现的最有前途的研究领域之一。目前，脂质的质谱分析涉及两种互补方法：直接进样（鸟枪法脂质组学）和液相色谱与质谱联用。在本章中，我将展示使用电喷雾电离串联质谱的鸟枪法脂质组学方法，用于直接从组织或体液的粗生物提取物中分析脂质分子种类。
