通过电喷雾电离质谱法对脂质种类进行准确量化——脂质组学面临的一项关键挑战。
Accurate quantification of lipid species by electrospray ionization mass spectrometry - Meet a key challenge in lipidomics.
作者信息
Yang Kui, Han Xianlin
机构信息
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110 USA.
出版信息
Metabolites. 2011 Dec 1;1(1):21-40. doi: 10.3390/metabo1010021. Epub 2011 Nov 11.
Abstract
Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met.
摘要
电喷雾电离质谱（ESI-MS）已成为脂质组学中鉴定和定量单个脂质种类最受欢迎且最强大的技术之一。与此同时，通过ESI-MS对脂质种类进行定量分析也已成为脂质组学面临挑战时的一个主要障碍。在此，我们讨论基于不同质谱方法进行脂质定量的原理、优势及可能存在的局限性，以及对准确量化单个脂质种类很重要的一些实际问题。因此，脂质组学的关键挑战之一——准确量化单个脂质种类，在实际中是可以实现的。
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2
Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses.基于多维质谱的 shotgun 脂质组学和脂质组学分析的新策略。
Mass Spectrom Rev. 2012 Jan-Feb;31(1):134-78. doi: 10.1002/mas.20342. Epub 2011 Jul 13.
3
Electrospray ionization tandem mass spectrometry (ESI-MS/MS)-based shotgun lipidomics.基于电喷雾电离串联质谱（ESI-MS/MS）的鸟枪法脂质组学。
Methods Mol Biol. 2011;708:259-75. doi: 10.1007/978-1-61737-985-7_16.
4
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues.基于液相色谱-质谱联用（LC-MS）的脂质组学用于体液和组织研究。
Methods Mol Biol. 2011;708:247-57. doi: 10.1007/978-1-61737-985-7_15.
5
Lipidomics: new tools and applications.脂质组学：新工具与新应用。
Cell. 2010 Dec 10;143(6):888-95. doi: 10.1016/j.cell.2010.11.033.
6
Lipidomics analysis reveals efficient storage of hepatic triacylglycerides enriched in unsaturated fatty acids after one bout of exercise in mice.脂质组学分析揭示了小鼠一次性运动后富含不饱和脂肪酸的肝三酰甘油的有效储存。
PLoS One. 2010 Oct 13;5(10):e13318. doi: 10.1371/journal.pone.0013318.
7
Improved sensitivity mass spectrometric detection of eicosanoids by charge reversal derivatization.通过电荷反转衍生化提高二十烷酸类物质的质谱检测灵敏度。
Anal Chem. 2010 Aug 15;82(16):6790-6. doi: 10.1021/ac100720p.
8
Advances in mass spectrometry for lipidomics.脂质组学中质谱分析的进展。
Annu Rev Anal Chem (Palo Alto Calif). 2010;3:433-65. doi: 10.1146/annurev.anchem.111808.073705.
9
Lipidomics: coming to grips with lipid diversity.脂质组学：应对脂质多样性。
Nat Rev Mol Cell Biol. 2010 Aug;11(8):593-8. doi: 10.1038/nrm2934. Epub 2010 Jul 7.
10
Selective desorption/ionization of sulfatides by MALDI-MS facilitated using 9-aminoacridine as matrix.利用 9-氨基吖啶作为基质，通过 MALDI-MS 实现硫脂的选择性解吸/电离。
J Lipid Res. 2010 Jun;51(6):1599-609. doi: 10.1194/jlr.D004077. Epub 2010 Feb 1.
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