Yang Kui, Han Xianlin
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110 USA.
Metabolites. 2011 Dec 1;1(1):21-40. doi: 10.3390/metabo1010021. Epub 2011 Nov 11.
Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met.
电喷雾电离质谱（ESI-MS）已成为脂质组学中鉴定和定量单个脂质种类最受欢迎且最强大的技术之一。与此同时，通过ESI-MS对脂质种类进行定量分析也已成为脂质组学面临挑战时的一个主要障碍。在此，我们讨论基于不同质谱方法进行脂质定量的原理、优势及可能存在的局限性，以及对准确量化单个脂质种类很重要的一些实际问题。因此，脂质组学的关键挑战之一——准确量化单个脂质种类，在实际中是可以实现的。
