Timenetsky J
Departamento de Microbiologia, Universidade de São Paulo, Brasil.
Rev Saude Publica. 1990 Feb;24(1):47-50. doi: 10.1590/s0034-89101990000100008.
Five disinfectants for household use were advertised on television during 1988 and the first half of 1989. The products were tested by a qualitative (Use-Dilution with 10 carriers, a conventional and simplified, adapted method) and a qualitative, adapted method with a view to evaluating their antimicrobial activity. The active compounds of the products, according to their respective labels were: 1--Parachlorophenol (0-Benzil) 0.1%; 2--Eter 2.4.4' Chloro (III) 2' hydroxiphenylic 0.1%; 3--N-alkyl dimethylbenzyl ammonium chlorides, N-alkyl dimethylethybenzyl ammonium 50%-1.6%; 4--Formaldehyde 37% (0.3% solution); 5--No information. The microorganisms used were: Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442 and Salmonella choleraesuis ATCC 10708. In the qualitative method the pseudomonas strain was recovered from disinfectants 1, 2, and 3 and the salmonella strain from disinfectants 2 and 3. All disinfectants showed germicidal effect 5.0 (99.999%) of reduction) in 15 seconds against all strains. Disinfectant 3 was contaminated with Enterobacter sp to the order of 10(4) cells/ml. This contaminant was sensitive against disinfectants 1, 4 and 5, in the qualitative method and had a relative resistance to disinfectant 2 in the quantitative method.
1988年至1989年上半年，五种家用消毒剂在电视上做了广告。对这些产品进行了定性检测（采用含10种载体的使用稀释法，这是一种传统且简化的改良方法）以及一种定性改良方法，以评估其抗菌活性。根据各自标签，这些产品的活性成分分别为：1--对氯苯酚（邻苯偶姻）0.1%；2--2,4,4'-三氯-2'-羟基二苯醚0.1%；3--烷基二甲基苄基氯化铵、烷基二甲基乙基苄基氯化铵50%-1.6%；4--37%甲醛（0.3%溶液）；5--无相关信息。所使用的微生物为：金黄色葡萄球菌ATCC 6538、铜绿假单胞菌ATCC 15442和猪霍乱沙门氏菌ATCC 10708。在定性检测中，从消毒剂1、2和3中检出了铜绿假单胞菌菌株，从消毒剂2和3中检出了沙门氏菌菌株。所有消毒剂在15秒内对所有菌株均显示出杀菌效果（杀灭率达5.0，即99.999%）。消毒剂3被肠杆菌属污染，污染程度达10⁴个细胞/毫升。在定性检测中，这种污染物对消毒剂1、4和5敏感，在定量检测中对消毒剂2具有相对抗性。
