Malavé I, Escalona E, Perdomo Y, Pocino M, Malavé D, Arends T
Centro de Medicina Experimental, Instituto Venezolano de Investgaciones Cientificas, Caracas.
Acta Haematol. 1990;84(2):89-94. doi: 10.1159/000205035.
To study the capacity of peripheral blood mononuclear cells (PBMC) from patients with sickle cell disease to synthesize antibodies in vitro, the levels of IgM, IgG, and IgA were quantitated in supernatants of cultured PBMC from a group of asymptomatic adults with sickle cell disease and from normal controls. The rates of spontaneous synthesis of IgM were similar in nonstimulated cultures of PBMC from patients and controls, whereas the amounts of IgG and IgA produced spontaneously by nonstimulated lymphocytes from the patients were significantly greater than those from controls. Similar levels of IgM, IgG, and IgA were detected in the supernatants of cultures stimulated with pokeweed mitogen from patients and controls. Thus, the capacity of PBMC to respond in vitro to pokeweed mitogen was preserved in the patients. The enhanced spontaneous synthesis of IgG and IgA suggests the presence of chronic polyclonal activation of B cells and/or defective regulation of the production of antibodies.
为研究镰状细胞病患者外周血单个核细胞（PBMC）在体外合成抗体的能力，对一组无症状成年镰状细胞病患者及正常对照者培养的PBMC上清液中的IgM、IgG和IgA水平进行了定量分析。患者和对照者PBMC非刺激培养物中IgM的自发合成率相似，而患者非刺激淋巴细胞自发产生的IgG和IgA量显著高于对照者。用美洲商陆丝裂原刺激患者和对照者培养物的上清液中检测到相似水平的IgM、IgG和IgA。因此，患者PBMC体外对美洲商陆丝裂原的反应能力得以保留。IgG和IgA自发合成增强提示存在B细胞慢性多克隆激活和/或抗体产生调节缺陷。
