Famularo G, Giacomelli R, Alesse E, Cifone M G, Morrone S, Boirivant M, Danese C, Perego M A, Santoni A, Tonietti G
Clinica Medica, University of L'Aquila, Italy.
J Clin Lab Immunol. 1989 Jun;29(2):59-63.
In 13 women affected by progressive systemic sclerosis (PSS) the activation state of circulating B lymphocytes by use of monoclonal antibodies and the in vitro IgG, IgA, IgM synthesis by peripheral blood mononuclear cells (PBMC) cultured with or without pokeweed-mitogen (PWM) were evaluated. The total and "activated" B lymphocytes were increased. The supernatants of PBMC cultured without PWM contained increased IgG and IgA levels; only in two patients, however, were IgM levels increased. The addition of PWM augmented IgG and IgA levels only in three cases and never IgM levels. Our results indicate that the polyclonal hyperactivity of B lymphocytes is a major immunologic feature in PSS patients. The lack of increase of in vitro immunoglobulin synthesis in the presence of PWM suggests either that B lymphocytes, already maximally activated in vivo, are unresponsive to further mitogen stimulation or that a more complex imbalance of B lymphocyte responsiveness to PWM may exist.
对13名进行性系统性硬化症（PSS）患者，评估了使用单克隆抗体时循环B淋巴细胞的激活状态，以及外周血单个核细胞（PBMC）在有或无商陆有丝分裂原（PWM）培养条件下体外IgG、IgA、IgM的合成情况。总的和“活化的”B淋巴细胞均增加。未添加PWM培养的PBMC上清液中IgG和IgA水平升高；然而，仅在两名患者中IgM水平升高。添加PWM仅在3例中使IgG和IgA水平升高，而从未使IgM水平升高。我们的结果表明，B淋巴细胞的多克隆高活性是PSS患者的主要免疫学特征。在有PWM存在时体外免疫球蛋白合成未增加，这表明要么体内已被最大程度激活的B淋巴细胞对进一步的有丝分裂原刺激无反应，要么可能存在B淋巴细胞对PWM反应性更复杂的失衡。
