伯氨喹体外研究：超氧自由基的形成及血红蛋白的氧化
Studies with primaquine in vitro: superoxide radical formation and oxidation of haemoglobin.
作者信息
Summerfield M, Tudhope G R
出版信息
Br J Clin Pharmacol. 1978 Oct;6(4):319-23. doi: 10.1111/j.1365-2125.1978.tb00858.x.
Abstract
- The production of superoxide radicals from primaquine diphosphate in aqueous solution has been demonstrated, using as indicator the reduction of cytochrome C with inhibition of the reaction by superoxide dismutase. 2. Primaquine-mediated oxidation of haemoglobin to methaemoglobin was reduced by the addition of catalase and increased by superoxide dismutase. Mannitol, a hydroxyl radical scavenger, abolished the increase in methaemoglobin observed in the presence of superoxide dismutase. EDTA reduced the oxidation of haemoglobin with and without superoxide dismutase. 3. Although the oxidation of haemoglobin in the presence of primaquine includes the effects of hydrogen peroxide, superoxide and hydroxyl radicals and metal ions, the results indicate that hydrogen peroxide, rather than the superoxide radical, is the main oxidizing species. The increase in haemoglobin oxidation occurring with superoxide dismutase may result from the augmented rate of hydrogen peroxide formation from superoxide radicals.
摘要
- 已证实在水溶液中，磷酸伯氨喹会产生超氧自由基，以细胞色素C的还原为指标，并发现超氧化物歧化酶可抑制该反应。2. 添加过氧化氢酶可降低伯氨喹介导的血红蛋白氧化为高铁血红蛋白的程度，而超氧化物歧化酶则会使其增加。甘露醇作为一种羟自由基清除剂，可消除在超氧化物歧化酶存在下观察到的高铁血红蛋白增加现象。无论有无超氧化物歧化酶，乙二胺四乙酸（EDTA）均可降低血红蛋白的氧化。3. 尽管在伯氨喹存在下血红蛋白的氧化包括过氧化氢、超氧自由基、羟自由基和金属离子的作用，但结果表明，过氧化氢而非超氧自由基是主要的氧化物种。超氧化物歧化酶存在时血红蛋白氧化的增加可能是由于超氧自由基生成过氧化氢的速率增加所致。
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