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人类精子中心体蛋白与生育能力的相关性

Correlation of human sperm centrosomal proteins with fertility.

作者信息

Hinduja Indira, Baliga Nishitha B, Zaveri Kusum

机构信息

Jaslok Hospital and Research Centre, Mehta Bhavan, Mumbai, India.

出版信息

J Hum Reprod Sci. 2010 May;3(2):95-101. doi: 10.4103/0974-1208.69344.

DOI:10.4103/0974-1208.69344
PMID:21209754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2970799/
Abstract

OBJECTIVE

The centrosome is the microtubule organizing center (MTOC) paternally inherited by the zygote during fertilization. As the centrosome is located in the midpiece of the sperm tail, we presume that oligoasthenozoospermic sperm samples should also have abnormal concentrations of centrosomal proteins. This study therefore aims to determine if there is any correlation between sperm centrosomal proteins, centrin, α and γ-tubulin, in sperm samples from normozoospermic and oligoasthenozoospermic men.

MATERIALS AND METHODS

Proteins were extracted from the normozoospermic and oligoasthenozoospermic sperm samples and analyzed by Western Blot and ELISA for centrin, α and γ-tubulin.

RESULTS

The levels of centrin, α and γ-tubulin are markedly lower in oligoasthenozoospermic sperm samples as compared to the normozoospermic sperm samples.

CONCLUSIONS

Lower centrosomal protein expression in sperm samples of oligoasthenozoospermic infertile males may be a possible cause for their reduced fertility status. Further studies on these proteins are warranted to design rational approaches for the diagnosis and treatment of male infertility.

摘要

目的

中心体是受精过程中由受精卵父系遗传的微管组织中心（MTOC）。由于中心体位于精子尾部的中段，我们推测少弱精子症患者的精子样本中中心体蛋白的浓度也应该异常。因此，本研究旨在确定正常精子症和少弱精子症男性的精子样本中精子中心体蛋白、中心蛋白、α和γ微管蛋白之间是否存在任何相关性。

材料与方法

从正常精子症和少弱精子症的精子样本中提取蛋白质，并通过蛋白质免疫印迹法和酶联免疫吸附测定法分析中心蛋白、α和γ微管蛋白。

结果

与正常精子症的精子样本相比，少弱精子症的精子样本中中心蛋白、α和γ微管蛋白的水平明显较低。

结论

少弱精子症不育男性精子样本中中心体蛋白表达较低可能是其生育能力下降的一个可能原因。有必要对这些蛋白质进行进一步研究，以设计出诊断和治疗男性不育症的合理方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/7b6514d1133b/JHRS-3-95-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/37c67342a036/JHRS-3-95-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/f1e50747c112/JHRS-3-95-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/6f136228f231/JHRS-3-95-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/3d1be4b83f7b/JHRS-3-95-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/78f11fe2dd58/JHRS-3-95-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/2e65a4cb860b/JHRS-3-95-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/7b6514d1133b/JHRS-3-95-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/37c67342a036/JHRS-3-95-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/f1e50747c112/JHRS-3-95-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/6f136228f231/JHRS-3-95-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/3d1be4b83f7b/JHRS-3-95-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/78f11fe2dd58/JHRS-3-95-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/2e65a4cb860b/JHRS-3-95-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f633/2970799/7b6514d1133b/JHRS-3-95-g007.jpg

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