Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
Int J Med Sci. 2010 Dec 27;8(1):16-29. doi: 10.7150/ijms.8.16.
Hemopoietic system derived progenitor cells with mesenchymal features have been identified including CD14(+) monocyte-derived progenitors. However, it is unclear whether there are mesenchyme derived progenitors with hematopoietic potential. Herein, we identified a novel CD14(-) cell-derived population with both mesenchymal and hematopoietic features in rat peripheral blood, and this cell population is different from the CD14(+) monocyte-derived progenitors but designated peripheral blood multipotential mesenchymal progenitors (PBMMPs). Phenotype analysis demonstrated expression of mesenchymal markers in PBMMPs including BMPRs, Endoglin/CD105, Fibronectin (Fn), Vimentin (Vim), Collagen (Col) I/II/III along with hematopoietic marker CD34. CD14(+) cell-derived population shared the same characteristics with CFs. In mixed culture of CD14(+) and CD14(-) cells, PBMMPs were a predominant component and expressed CD29(high), CD73(high), CD34(high), CD45(low) and CD90. Except for the value of mixed T lymphocytes and CD14(+) cell-derived population, hematopoietic characters of cultured PBMMPs were indicated by CD14(-)/CD34(+)/CD45(-)/CD90(+). The mesenchymal origin was further confirmed by comparing PBMMPs with bone marrow stromal cells. Finally, we transplanted PBMMPs into a skin wound model, and results showed the specific potential of PBMMPs in not only extracellular matrix secretion but epidermal regeneration. This study provides evidence that peripheral blood contains common hematopoietic-mesenchymal progenitors from both hematopoietic and mesenchymal lineages, and CD34(+) mesenchymal progenitors are a possible alternative source of epidermal cells in wound healing.
造血系统来源的具有间充质特征的祖细胞已被鉴定，包括 CD14(+)单核细胞衍生的祖细胞。然而，目前尚不清楚是否存在具有造血潜能的间质来源的祖细胞。在此，我们在大鼠外周血中鉴定出一种新型的 CD14(-)细胞衍生的具有间充质和造血特征的细胞群体，该细胞群体不同于 CD14(+)单核细胞衍生的祖细胞，但被指定为外周血多能间充质祖细胞（PBMMP）。表型分析表明，PBMMP 表达间充质标志物，包括 BMPRs、Endoglin/CD105、纤连蛋白（Fn）、波形蛋白（Vim）、胶原蛋白（Col）I/II/III 以及造血标志物 CD34。CD14(+)细胞衍生的群体与 CFs 具有相同的特征。在 CD14(+)和 CD14(-)细胞的混合培养中，PBMMP 是主要成分，表达 CD29(high)、CD73(high)、CD34(high)、CD45(low)和 CD90。除了混合 T 淋巴细胞和 CD14(+)细胞衍生群体的值外，培养的 PBMMP 的造血特征还通过 CD14(-)/CD34(+)/CD45(-)/CD90(+)来表示。通过将 PBMMP 与骨髓基质细胞进行比较，进一步证实了其间充质来源。最后，我们将 PBMMP 移植到皮肤创伤模型中，结果表明 PBMMP 不仅具有细胞外基质分泌的特异性潜能，而且具有表皮再生的特异性潜能。这项研究提供了证据，表明外周血中含有来自造血和间充质谱系的共同造血-间充质祖细胞，CD34(+)间充质祖细胞可能是创伤愈合中表皮细胞的一种替代来源。
