Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
PLoS One. 2010 Dec 29;5(12):e15713. doi: 10.1371/journal.pone.0015713.
The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL-1 and Mexico A), T. p. pertenue strains (Samoa D, CDC-2 and Gauthier), and the Fribourg-Blanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate sizes of the genomes investigated based on this whole genome fingerprinting (WGF) analysis ranged from 1139.3-1140.4 kb, with the estimated genome sequence identity of 99.57-99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. The unclassified simian Fribourg-Blanc isolate was more closely related to T. p. pertenue than to T. p. pallidum strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains. These regions of sequence divergence could be used for the molecular detection and discrimination of syphilis and yaws strains.
八种密螺旋体的基因组，包括梅毒螺旋体菌株（Nichols、SS14、DAL-1 和 Mexico A）、雅司螺旋体菌株（Samoa D、CDC-2 和 Gauthier）和 Fribourg-Blanc 分离株，被扩增为 133 个重叠的扩增子，并比较了这些片段的限制模式。根据全基因组指纹图谱（WGF）分析，所研究的基因组的大致大小范围为 1139.3-1140.4kb，在同源基因组区域的估计基因组序列同一性为 99.57-99.98%。限制目标位点分析，检测到参考 Nichols 基因组中存在的 1773 个单个限制位点，揭示了所有菌株的高基因组结构相似性。未分类的猿猴 Fribourg-Blanc 分离株与雅司螺旋体比与梅毒螺旋体菌株更为密切相关。梅毒螺旋体和雅司螺旋体菌株之间的大多数遗传差异积累在六个基因组区域。这些基因组差异可能导致梅毒螺旋体和雅司螺旋体菌株之间观察到的致病性差异。这些序列差异区域可用于梅毒和雅司病菌株的分子检测和鉴别。
