Besson F, Hourdou M L, Michel G
Laboratoire de Biochimie Microbienne, Université Claude Bernard, Villeurbanne, France.
Biochim Biophys Acta. 1990 Nov 9;1036(2):101-6. doi: 10.1016/0304-4165(90)90020-w.
The biosynthesis of iturin, an antibiotic containing a beta-amino fatty acid, was studied by incubating Bacillus subtilis in the presence of various 14C-labelled precursors. Sodium acetate or palmitic acid were incorporated into the beta-amino acids of iturin. Among the alpha-amino acids (asparagine, glutamine, serine, proline and tyrosine) in the peptidic part of iturin, asparagine appears to be the best precursor. In the presence of sodium [14C]acetate or [14C]asparagine, there was a synthesis of radioactive compound (compound X) before the synthesis of radioactive iturin. Compound X contained asparagine and/or aspartic acid, glutamine and/or glutamic acid and beta-hydroxy fatty acids.
通过在各种¹⁴C标记的前体存在下培养枯草芽孢杆菌，研究了iturin（一种含有β-氨基脂肪酸的抗生素）的生物合成。乙酸钠或棕榈酸被掺入iturin的β-氨基酸中。在iturin肽部分的α-氨基酸（天冬酰胺、谷氨酰胺、丝氨酸、脯氨酸和酪氨酸）中，天冬酰胺似乎是最佳前体。在[¹⁴C]乙酸钠或[¹⁴C]天冬酰胺存在下，在放射性iturin合成之前有放射性化合物（化合物X）的合成。化合物X含有天冬酰胺和/或天冬氨酸、谷氨酰胺和/或谷氨酸以及β-羟基脂肪酸。
