Departamento de Zootecnia, Universidade Federal de Viçosa, Viçosa, Brazil.
J Anim Breed Genet. 2011 Feb;128(1):28-34. doi: 10.1111/j.1439-0388.2010.00887.x. Epub 2010 Oct 28.
Seventy-two pigs of three genetic groups (Brazilian indigenous breed Piau, Commercial line and Crossbred) of both sexes were slaughtered at four live weights (30, 60, 90 and 120 kg). Intramuscular fat (IMF) content in Longissimus dorsi muscle of each animal was extracted and correlated with candidate gene mRNA expression (ATN1, EEF1A2, FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1). Within slaughter weight of 120 kg, Piau and Crossbred pigs showed higher IMF content (p < 0.05) than commercial animals, with 2.48, 2.08 and 1.00% respectively. Barrows presented higher values of IMF (p < 0.05) than gilts (1.54 and 1.30% respectively). Gene expression of EEF1A2, FABP3, LDLR, OBSCN, PDHB, TRDN and RYR1 were correlated with IMF (p < 0.05) using the whole dataset. For Piau data only, expression of FABP3, LDLR, MGP, OBSCN, PDHB, TRDN and RYR1 showed correlation with IMF (p < 0.05). Genes that have important roles in lipid transportation inside the cell (FABP3) and tissues (LDLR) showed correlation with IMF of, respectively, 0.68 and 0.63 using the whole data set, and 0.90 and 0.91 using data from Piau animals. The highly positive correlation of the LDLR and FAPB3 expression with IMF content may confirm that these genes are important for fat deposition in the porcine L. dorsi muscle.
七十二头猪（三个遗传群体：巴西本土品种皮奥、商业品种和杂交品种），雌雄各半，在四个活体体重（30、60、90 和 120 公斤）时屠宰。从每个动物的背最长肌中提取肌内脂肪（IMF）含量，并与候选基因 mRNA 表达（ATN1、EEF1A2、FABP3、LDLR、MGP、OBSCN、PDHB、TRDN 和 RYR1）相关联。在 120 公斤的屠宰体重内，皮奥和杂交猪的 IMF 含量高于商业猪（分别为 2.48%、2.08%和 1.00%），公猪的 IMF 值高于母猪（分别为 1.54%和 1.30%）。EEF1A2、FABP3、LDLR、OBSCN、PDHB、TRDN 和 RYR1 的基因表达与 IMF 相关（p < 0.05），整个数据集都如此。仅对皮奥的数据而言，FABP3、LDLR、MGP、OBSCN、PDHB、TRDN 和 RYR1 的表达与 IMF 相关（p < 0.05）。在细胞内（FABP3）和组织（LDLR）中对脂质运输有重要作用的基因与 IMF 分别具有 0.68 和 0.63 的相关性，使用整个数据集，以及与皮奥动物数据则分别具有 0.90 和 0.91 的相关性。LDLR 和 FAPB3 表达与 IMF 含量的高度正相关可能证实了这些基因对猪背最长肌脂肪沉积的重要性。
