Oral Microbiology, School of Dentistry, Cardiff University, Heath Park, Cardiff, CF14 4XY.
Br Dent J. 2011 Jan 8;210(1):E22. doi: 10.1038/sj.bdj.2010.1224.
Citrox is a formulation of soluble bioflavonoids obtained from citrus fruits. The non-toxic and antimicrobial properties of natural bioflavonoids are well documented, and consequently there has been interest in the therapeutic application of these substances.
To determine the antimicrobial activity of two Citrox formulations (BC30 and MDC30) with different bioflavonoid combinations against a range of oral microorganisms.
The antimicrobial activity of both formulations was tested against 14 bacterial species and six Candida species. The two Citrox formulations (dilution range 0.007-8% v/v) were firstly evaluated by determining the in vitro Minimal Inhibitory Concentration (MIC) against planktonic microorganisms in a broth microdilution assay. Secondly, the ability of the same serial dilutions to inhibit microbial growth was assessed in a modified microtitre biofilm assay.
Both Citrox formulations exhibited antimicrobial activity. The BC30 formulation demonstrated greater activity than MDC30 and significantly inhibited growth of all bacterial species and most candidal species tested at a concentration of 1% (v/v) in both the broth and the biofilm assay.
Bioflavonoid preparations of Citrox have a broad-spectrum of antimicrobial activity against oral microorganisms, and as such have the potential to be used within therapeutic preparations for the control of the oral microflora.
Citrox 是一种从柑橘类水果中提取的可溶性生物类黄酮配方。天然生物类黄酮的无毒和抗菌特性已有充分记录，因此人们对这些物质的治疗应用产生了兴趣。
确定两种具有不同生物类黄酮组合的 Citrox 配方（BC30 和 MDC30）对一系列口腔微生物的抗菌活性。
用两种 Citrox 配方（浓度范围 0.007-8%v/v）通过肉汤微量稀释法测定浮游微生物的体外最小抑菌浓度（MIC）来测试两种配方的抗菌活性。其次，在改良的微量滴定板生物膜测定中评估相同系列稀释液抑制微生物生长的能力。
两种 Citrox 配方均表现出抗菌活性。BC30 配方的活性大于 MDC30，并且在肉汤和生物膜测定中，浓度为 1%（v/v）时，均显著抑制所有测试的细菌和大多数念珠菌的生长。
Citrox 的生物类黄酮制剂对口腔微生物具有广谱抗菌活性，因此有可能在用于控制口腔微生物群的治疗制剂中使用。
