Laboratório de Química, Bioquímica e Biologia Molecular de Alimentos, Departamento de Alimentos e Nutrição Experimental, FCF, Universidade de São Paulo, 05508-000 São Paulo, SP, Brazil.
J Sci Food Agric. 2011 Feb;91(3):523-31. doi: 10.1002/jsfa.4216. Epub 2010 Nov 22.
This study reported the effects of the daily intake of anthocyanins and ellagitannins (ET) extracted from blackberries on the markers for oxidative status in healthy rats.
The phenolic compounds were administered from three different extracts: an aqueous extract of blackberry (BJ) and its two derived fractions: anthocyanin-enriched (AF) and ET-enriched (EF) fractions. After 35 days’ administration, the AF and EF extracts significantly reduced thiobarbituric acid reactive substance levels and increased glutathione levels in the liver,kidney and brain. Plasma antioxidant capacity increased only in the group that received AF. Antioxidant enzyme activity and expression did not follow a pattern of response varying according to the tissues and extracts. A significant increase in the catalase activity was observed only in the plasma of the groups administered anthocyanin-containing extracts,which were the BJ and AF groups. Glutathione peroxidase activity was significantly increased in the liver and brain after EF treatment, and the highest increase in its expression was observed in the livers and brains of rats that received AF and EF, respectively.
The results demonstrate that long-term intake of anthocyanin and ET through diet affects antioxidant enzymeactivity and expression, and enhances oxidative markers in healthy rats.
本研究报告了每日摄入黑莓中提取的花青素和鞣花单宁（ET）对健康大鼠氧化状态标志物的影响。
这些酚类化合物来自三种不同的提取物：黑莓的水提取物（BJ）及其两个衍生部分：花青素富集（AF）和 ET 富集（EF）部分。经过 35 天的给药后，AF 和 EF 提取物显著降低了肝脏、肾脏和大脑中的丙二醛含量，增加了谷胱甘肽含量。只有接受 AF 提取物的组血浆抗氧化能力增加。抗氧化酶活性和表达没有表现出根据组织和提取物变化的反应模式。只有在给予含花青素的提取物的组（BJ 和 AF 组）的血浆中观察到过氧化氢酶活性的显著增加。EF 处理后，肝脏和大脑中的谷胱甘肽过氧化物酶活性显著增加，AF 和 EF 组的大鼠肝脏和大脑中观察到其表达的最高增加。
这些结果表明，长期通过饮食摄入花青素和 ET 会影响抗氧化酶活性和表达，并增强健康大鼠的氧化标志物。
