College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 136-701, Republic of Korea.
J Agric Food Chem. 2011 Nov 9;59(21):11786-93. doi: 10.1021/jf104192a. Epub 2011 Oct 7.
Anthocyanins were extracted from the fruits of Rubus coreanus. Whether their antioxidant properties and antiulcer activity in gastric ulceration have been accompanied by the activation of matrix metalloproteainse-2 (MMP-2) was investigated. To assess the effect of anthocyanins on gastric ulcer, the rats were administered with anthocyanins (20, 50, and 80 mg/kg of body weight) before treatment with naproxen (80 mg/kg of body weight) to induce gastric ulceration. Lipid peroxidation and the activities of radical scavenging enzymes such as catalase, superoxide dismutase, and glutathione peroxidase were determined. The MMP-2 level was tested by zymography and Western blot. Anthocyanins of R. coreanus exhibit possible antiulcer activity in acute ulcer in a rat model by preventing lipid peroxidation and a significant increase in the activities of antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase. Also, anthocyanins induce activation of MMP-2 and attenuate the activity of the proinflammatory molecules, such as tumor necrosis factor-α and interleukin-1β.
从覆盆子果实中提取花色苷。研究了花色苷的抗氧化特性和在胃溃疡中的抗溃疡活性是否伴随着基质金属蛋白酶-2 (MMP-2)的激活。为了评估花色苷对胃溃疡的影响，在给予萘普生（80mg/kg 体重）诱导胃溃疡之前，用 20、50 和 80mg/kg 体重的花色苷对大鼠进行处理。测定脂质过氧化和自由基清除酶如过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。通过酶谱法和 Western blot 测定 MMP-2 水平。覆盆子花色苷通过防止脂质过氧化和显著增加过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶等抗氧化酶的活性，在大鼠急性溃疡模型中表现出可能的抗溃疡活性。此外，花色苷诱导 MMP-2 的激活，并减弱促炎分子如肿瘤坏死因子-α和白细胞介素-1β的活性。
