干细胞自我更新和分化中的可变剪接。

Alternative splicing in stem cell self-renewal and diferentiation.

机构信息

Department of Cellular and Molecular Medicine, Stem Cell Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, California, 92037, USA.

出版信息

Adv Exp Med Biol. 2010;695:92-104. doi: 10.1007/978-1-4419-7037-4_7.

DOI:10.1007/978-1-4419-7037-4_7

PMID:21222201

Abstract

This chapter provides a review of recent advances in understanding the importance of alternative pre-messenger RNA splicing in stem cell biology. The majority of transcribed pre-mRNAs undergo RNA splicing where introns are excised and exons are juxtaposed to form mature messenger RNA sequences. This regulated, selective removal of whole or portions of exons by alternative splicing provides avenues for control of RNA abundance and proteome diversity. We discuss several examples of key alternative splicing events in stem cell biology and provide an overview of recently developed microarray and sequencing technologies that enable systematic and genome-wide assessment of the extent of alternative splicing during stem cell differentiation.

摘要

这一章回顾了近年来在理解干细胞生物学中选择性前 mRNA 剪接的重要性方面的进展。大多数转录的前 mRNA 都经历 RNA 剪接，其中内含子被切除，外显子被并置形成成熟的信使 RNA 序列。这种通过选择性剪接对整个或部分外显子的有调控、有选择的去除为控制 RNA 丰度和蛋白质组多样性提供了途径。我们讨论了干细胞生物学中几个关键的选择性剪接事件的例子，并概述了最近开发的微阵列和测序技术，这些技术可以系统地和全基因组地评估干细胞分化过程中选择性剪接的程度。