Institute of Systems Biology & Bioinformatics, National Central University, No. 300, Chung-da Rd., Chung-li 32001, Taiwan, ROC.
Bioresour Technol. 2011 Mar;102(5):4232-40. doi: 10.1016/j.biortech.2010.12.067. Epub 2010 Dec 22.
Eighteen 4-t-octylphenol-degrading bacteria were isolated and screened for the presence of degradative genes by polymerase chain reaction method using four designed primer sets. The primer sets were designed to amplify specific fragments from multicomponent phenol hydroxylase, single component monooxygenase, catechol 1,2-dioxygenase and catechol 2,3-dioxygenase genes. Seventeen of the 18 isolates exhibited the presence of a 232 bp amplicon that shared 61-92% identity to known multicomponent phenol hydroxylase gene sequences from short and/or medium-chain alkylphenol-degrading strains. Twelve of the 18 isolates were positive for a 324 bp region that exhibited 78-95% identity to the closest published catechol 1,2-dioxygenase gene sequences. The two strains, Pseudomonas putida TX2 and Pseudomonas sp. TX1, contained catechol 1,2-dioxygenase genes also have catechol 2,3-dioxygenase genes. Our result revealed that most of the isolated bacteria are able to degrade long-chain alkylphenols via multicomponent phenol hydroxylase and the ortho-cleavage pathway.
采用聚合酶链反应（PCR）方法，使用四组设计引物，从 18 株 4-辛基酚降解菌中筛选出具有降解基因的菌株。这些引物组被设计用来扩增多组分苯酚羟化酶、单组分单加氧酶、儿茶酚 1,2-双加氧酶和儿茶酚 2,3-双加氧酶基因的特定片段。在 18 株分离菌中，有 17 株菌表现出 232bp 的扩增子，与短链和/或中链烷基酚降解菌的已知多组分苯酚羟化酶基因序列具有 61-92%的同源性。在 18 株分离菌中，有 12 株菌的 324bp 区域与最近发表的儿茶酚 1,2-双加氧酶基因序列具有 78-95%的同源性。这两株菌，即恶臭假单胞菌 TX2 和假单胞菌 TX1，含有儿茶酚 1,2-双加氧酶基因，也含有儿茶酚 2,3-双加氧酶基因。我们的研究结果表明，大多数分离到的细菌能够通过多组分苯酚羟化酶和邻位裂解途径降解长链烷基酚。
