Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, 23 Riia Street, Tartu 51010, Estonia.
Syst Appl Microbiol. 2013 Dec;36(8):525-32. doi: 10.1016/j.syapm.2013.07.004. Epub 2013 Sep 4.
A set of phenol-degrading strains of a collection of bacteria isolated from Baltic Sea surface water was screened for the presence of two key catabolic genes coding for phenol hydroxylases and catechol 2,3-dioxygenases. The multicomponent phenol hydroxylase (LmPH) gene was detected in 70 out of 92 strains studied, and 41 strains among these LmPH(+) phenol-degraders were found to exhibit catechol 2,3-dioxygenase (C23O) activity. Comparative phylogenetic analyses of LmPH and C23O sequences from 56 representative strains were performed. The studied strains were mostly affiliated to the genera Pseudomonas and Acinetobacter. However, the study also widened the range of phenol-degraders by including the genus Limnobacter. Furthermore, using a next generation sequencing approach, the LmPH genes of Limnobacter strains were found to be the most prevalent ones in the microbial community of the Baltic Sea surface water. Four different Limnobacter strains having almost identical 16S rRNA gene sequences (99%) and similar physiological properties formed separate phylogenetic clusters of LmPH and C23O genes in the respective phylogenetic trees.
从波罗的海表面水中分离的一组细菌的一组苯酚降解菌株被筛选，以检测编码苯酚羟化酶和儿茶酚 2,3-双加氧酶的两个关键代谢基因的存在。在所研究的 92 株菌中，检测到 70 株含有多组分苯酚羟化酶（LmPH）基因，其中 41 株 LmPH(+)苯酚降解菌表现出儿茶酚 2,3-双加氧酶（C23O）活性。对 56 株代表性菌株的 LmPH 和 C23O 序列进行了比较系统发育分析。研究的菌株主要属于假单胞菌属和不动杆菌属。然而，该研究还通过包括 Limnobacter 属，拓宽了苯酚降解菌的范围。此外，使用下一代测序方法，发现 Limnobacter 菌株的 LmPH 基因在波罗的海表面水中的微生物群落中最为普遍。具有几乎相同 16S rRNA 基因序列（99%）和相似生理特性的 4 株不同 Limnobacter 菌株在各自的系统发育树中形成了 LmPH 和 C23O 基因的单独聚类。
