Anderson G W, Slayter M V, Hall W, Peters C J
Disease Assessment Division, USAMRIID, Ft. Detrick, Frederick, Maryland.
Arch Virol. 1990;114(3-4):203-12. doi: 10.1007/BF01310749.
The hamster, Mesocricetus auratus, was examined as a possible model for investigating the poorly defined pathogenesis of the family Bunyaviridae, genus Phlebovirus. Punta Toro virus (PTV) isolates from Eastern Panama were highly virulent for two outbred and five inbred hamster strains, while isolates from western Panama were of low virulence. The Adames strain (eastern Panama) of PTV (LD50 approximately 1 PFU, sc) caused an acute fatal disease (average survival time, 3.8 days) in 10-week-old Lak: LVG (SYR) hamsters. Severe necrosis of the liver, spleen, and small intestine was associated with extensive expression of viral antigen in these organs. The Balliet strain (western Panama) of PTV (LD50 greater than 6 log10 PFU, subcutaneously) caused a mild hepatocellular infection with peak viral liver titers of 3-4 log10 PFU/g compared to 8-9 log10 PFU/g for the Adames strain. We observed histological lesions in the red pulp of the spleen or the lamina propria of the small intestine with the Adames strain. Lesions in the hamsters had characteristics of disseminated intravascular coagulation (DIC). The PTV-hamster model shares similarities to Rift Valley fever (phleboviral disease), which causes fatal disease in man and domesticated ruminants.
叙利亚仓鼠（Mesocricetus auratus）被作为一种可能的模型，用于研究布尼亚病毒科白蛉病毒属发病机制尚不明确的情况。来自巴拿马东部的蓬塔托罗病毒（PTV）分离株对两个远交系和五个近交系仓鼠品系具有高致病性，而来自巴拿马西部的分离株致病性较低。PTV的阿达姆斯株（来自巴拿马东部）（半数致死剂量约为1个空斑形成单位，皮下接种）在10周龄的Lak:LVG（SYR）仓鼠中引发急性致命疾病（平均存活时间为3.8天）。肝脏、脾脏和小肠的严重坏死与这些器官中病毒抗原的大量表达有关。PTV的巴利埃特株（来自巴拿马西部）（半数致死剂量大于6 log10空斑形成单位，皮下接种）引起轻度肝细胞感染，病毒在肝脏中的峰值滴度为3 - 4 log10空斑形成单位/克，而阿达姆斯株为8 - 9 log10空斑形成单位/克。我们观察到，感染阿达姆斯株的仓鼠脾脏红髓或小肠固有层出现组织学病变。仓鼠的病变具有弥散性血管内凝血（DIC）的特征。PTV - 仓鼠模型与裂谷热（白蛉病毒性疾病）有相似之处，裂谷热会在人类和家养反刍动物中引发致命疾病。
