Ogura T, Takenouchi N, Yamaguchi M, Matsukage A, Sugimura T, Esumi H
Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Oct 30;172(2):377-84. doi: 10.1016/0006-291x(90)90683-e.
The expression level of poly(ADP-ribose) polymerase mRNA as well as the level of enzymatic activity were examined in various mouse organs by northern blot and activity gel analyses. High levels of the mRNA expression and enzymatic activity were observed in testis, thymus, spleen, and brain. On the other hand, low levels of the mRNA expression and enzymatic activity were observed in liver and kidney. These findings suggest that the expression of the poly(ADP-ribose) polymerase is mainly regulated by transcription. Striking similarity was observed between the patterns of organ distribution of enzymatic activities of poly(ADP-ribose) polymerase and DNA polymerase beta in various mouse organs.
通过Northern印迹和活性凝胶分析检测了聚（ADP - 核糖）聚合酶mRNA的表达水平以及酶活性水平。在睾丸、胸腺、脾脏和大脑中观察到高水平的mRNA表达和酶活性。另一方面，在肝脏和肾脏中观察到低水平的mRNA表达和酶活性。这些发现表明聚（ADP - 核糖）聚合酶的表达主要受转录调控。在各种小鼠器官中，聚（ADP - 核糖）聚合酶和DNA聚合酶β的酶活性器官分布模式之间观察到惊人的相似性。
