XRCC1多肽与DNA聚合酶β以及可能的聚（ADP - 核糖）聚合酶相互作用，并且DNA连接酶III在体外是一种新型分子“切口传感器”。
XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro.
作者信息
Caldecott K W, Aoufouchi S, Johnson P, Shall S
机构信息
Zeneca Laboratory for Cell and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
出版信息
Nucleic Acids Res. 1996 Nov 15;24(22):4387-94. doi: 10.1093/nar/24.22.4387.
Abstract
The DNA repair proteins XRCC1 and DNA ligase III are physically associated in human cells and directly interact in vitro and in vivo. Here, we demonstrate that XRCC1 is additionally associated with DNA polymerase-beta in human cells and that these polypeptides also directly interact. We also present data suggesting that poly (ADP-ribose) polymerase can interact with XRCC1. Finally, we demonstrate that DNA ligase III shares with poly (ADP-ribose) polymerase the novel function of a molecular DNA nick-sensor, and that the DNA ligase can inhibit activity of the latter polypeptide in vitro. Taken together, these data suggest that the activity of the four polypeptides described above may be co-ordinated in human cells within a single multiprotein complex.
摘要
DNA修复蛋白XRCC1和DNA连接酶III在人类细胞中存在物理关联，并且在体外和体内都能直接相互作用。在此，我们证明XRCC1在人类细胞中还与DNA聚合酶β相关联，且这些多肽也能直接相互作用。我们还提供数据表明聚（ADP - 核糖）聚合酶可与XRCC1相互作用。最后，我们证明DNA连接酶III与聚（ADP - 核糖）聚合酶具有分子DNA切口传感器的新功能，并且该DNA连接酶在体外可抑制后一种多肽的活性。综上所述，这些数据表明上述四种多肽的活性可能在人类细胞内的单个多蛋白复合物中得到协调。
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