Carpenter K L, Ballantine J A, Fussell B, Enright J H, Mitchinson M J
Department of Pathology, University of Cambridge, U.K.
Atherosclerosis. 1990 Aug;83(2-3):217-29. doi: 10.1016/0021-9150(90)90167-h.
The amount of cholest-5-en-3 beta,7 beta-diol (CD) was significantly higher in cultures of human monocytes incubated with cholesteryl linoleate-bovine serum albumin (CL/BSA) artificial lipoproteins than in no-cell control incubations of CL/BSA. CD production by monocytes was almost completely inhibited by the radical scavengers butylated hydroxytoluene (BHT), probucol, and alpha-tocopherol, and was partially inhibited by the metal chelator EDTA. The production of CD was accompanied by decrease in linoleic acid. CD amounts were negligible in incubations of monocytes with cholesteryl oleate/BSA (CO/BSA) or cholesterol/BSA (C/BSA). Ability to produce CD from CL/BSA appeared to increase with age in culture of human monocyte-macrophages. Considerable variations were observed in the CD production from CL/BSA by monocytes from different blood donations. Higher levels of CD production appeared more common with monocytes from men than from women. The significance of these results in the context of human atherosclerosis is discussed.
与胆固醇亚油酸酯 - 牛血清白蛋白（CL/BSA）人工脂蛋白一起孵育的人单核细胞培养物中，胆甾 - 5 - 烯 - 3β,7β - 二醇（CD）的含量显著高于CL/BSA的无细胞对照孵育物。单核细胞产生CD的过程几乎完全被自由基清除剂丁基化羟基甲苯（BHT）、普罗布考和α - 生育酚抑制，并且部分被金属螯合剂EDTA抑制。CD的产生伴随着亚油酸的减少。单核细胞与胆固醇油酸酯/牛血清白蛋白（CO/BSA）或胆固醇/牛血清白蛋白（C/BSA）孵育时，CD的量可忽略不计。在人单核细胞 - 巨噬细胞培养物中，从CL/BSA产生CD的能力似乎随着年龄增长而增加。不同献血者的单核细胞从CL/BSA产生CD的情况存在相当大的差异。男性单核细胞产生较高水平的CD似乎比女性更常见。本文讨论了这些结果在人类动脉粥样硬化背景下的意义。
