Wicher K, Wicher V, Nakeeb S M, Dubiski S
Br J Vener Dis. 1983 Dec;59(6):349-58. doi: 10.1136/sti.59.6.349.
Rabbit testes were injected with suspensions of Treponema pallidum, washed T pallidum, heat killed T pallidum, or Reiter treponemes. The testes were removed three to 24 days after injection and examined for the number of treponemes, the presence of treponemal antibodies, histopathological changes, and presence of T and B cells. In animals infected with T pallidum a substantial number (10(6)-10(7)/ml) of organisms were still present at day 24 in spite of early local production of antibodies and increasing infiltration with plasma cells, T lymphocytes, and macrophages. In animals infected with washed T pallidum a lower degree of inflammation was observed than in those infected with unmodified T pallidum, and the treponemal antibodies were detected simultaneously in samples of testicular fluid and serum. In the groups injected with heat killed T pallidum and Reiter treponemes no macroscopical or microscopical changes were detected, although in the group injected with heat killed T pallidum treponemal antibodies were detected in the testicular fluid on day 24.
将梅毒螺旋体、洗涤后的梅毒螺旋体、热灭活的梅毒螺旋体或赖特螺旋体的悬液注射到兔睾丸中。注射后3至24天取出睾丸，检查螺旋体数量、螺旋体抗体的存在情况、组织病理学变化以及T细胞和B细胞的存在情况。在感染梅毒螺旋体的动物中，尽管早期局部产生了抗体，并且浆细胞、T淋巴细胞和巨噬细胞的浸润增加，但在第24天时仍有大量（10⁶ - 10⁷/ml）的病原体存在。在感染洗涤后梅毒螺旋体的动物中，观察到的炎症程度低于感染未处理梅毒螺旋体的动物，并且在睾丸液和血清样本中同时检测到螺旋体抗体。在注射热灭活梅毒螺旋体和赖特螺旋体的组中，未检测到宏观或微观变化，尽管在注射热灭活梅毒螺旋体的组中，在第24天时在睾丸液中检测到了螺旋体抗体。
