验证组织微阵列技术在恶性胸膜间皮瘤中的应用。

Validation of tissue microarray technology in malignant pleural mesothelioma.

机构信息

Asbestos Diseases Research Institute, Bernie Banton Centre, Concord, Australia.

出版信息

Pathology. 2011 Feb;43(2):128-32. doi: 10.1097/PAT.0b013e328342016c.

DOI:10.1097/PAT.0b013e328342016c

PMID:21233673

Abstract

AIMS

Tissue microarray (TMA) technology has been utilised for assessment of cancers including malignant pleural mesothelioma (MPM). Given the intralesional heterogeneity of MPM, it is questionable if TMAs can adequately represent MPMs. We here investigate the validity of TMAs for MPM.

METHODS

TMAs were constructed from at least five cores for each of 80 archival tumours processed by two centres between 1994 and 2009. The percentage of cases correctly subtyped on TMAs compared with whole sections, in relation to the number of cores analysed, was calculated. Immunohistochemical labelling for calretinin and D2-40 was performed on TMAs and whole sections. To evaluate the validity of quantitative immunohistochemistry, percentages of positive cells were recorded and two-way analysis of variance (ANOVA) performed.

RESULTS

Five cores were assessable for 91% of patients. Four cores were sufficient to reach concordance with the whole-section result in 98% of cases for calretinin and 99% for D2-40. The correlation of the quantitative scores between the whole section and TMA cores was statistically significant (D2-40, rho = 0.84, p < 2.2e-16; calretinin, rho = 0.65, p = 7.9e-11). Neither the origin nor age of the blocks affected the results.

CONCLUSION

If a minimum of four cores is used, TMA is an appropriate method for immunohistochemistry in MPM.

摘要

目的

组织微阵列（TMA）技术已被用于评估包括恶性胸膜间皮瘤（MPM）在内的癌症。鉴于 MPM 的瘤内异质性，TMA 是否能充分代表 MPM 存在疑问。我们在此研究 TMA 在 MPM 中的有效性。

方法

从 1994 年至 2009 年间由两个中心处理的 80 个存档肿瘤中，每个肿瘤至少构建了 5 个核心的 TMA。计算 TMA 相对于分析的核心数量与全切片相比正确分型的病例百分比。对 TMA 和全切片进行钙视网膜蛋白和 D2-40 的免疫组织化学标记。为了评估定量免疫组化的有效性，记录阳性细胞的百分比，并进行双向方差分析（ANOVA）。

结果

91%的患者可评估 5 个核心。4 个核心足以在 98%的病例中与全切片结果达成一致，钙视网膜蛋白为 99%，D2-40 为 99%。全切片和 TMA 核心之间的定量评分相关性具有统计学意义（D2-40，rho = 0.84，p < 2.2e-16；钙视网膜蛋白，rho = 0.65，p = 7.9e-11）。组织块的来源和年龄均不影响结果。