Durnev A D, Solomina A S, Daugel-Dauge N O, Zhanataev A K, Shreder E D, Nemova E P, Shreder O V, Veligura V A, Osminkina L A, Timoshenko V Yu, Seredenin S B
Institute of Pharmacology, Russian Academy of Medical Sciences, Russia.
Bull Exp Biol Med. 2010 Oct;149(4):445-9. doi: 10.1007/s10517-010-0967-3.
Silicon crystal 2-5 nm nanoparticles in the form of 1-5-μ granules in water suspension were injected intraperitoneally in a single dose to male F(1)(CBA×C57Bl/6) mice or to outbred albino rats on days 1, 7, and 14 of gestation. Silicon crystal nanoparticles in doses of 5, 25, and 50 mg/kg exhibited no cytogenetic activity in mouse bone marrow cells after 24-h exposure and in doses of 5 and 25 mg/kg after 7 and 14-day exposure. A 24-h exposure to silicon nanoparticles in a dose of 5 mg/kg significantly increased DNA damage (detected by DNA comet assay) in bone marrow cells. In a dose of 50 mg/kg they considerably increased DNA damage in bone marrow and brain cells after exposure of the same duration. Silicon nanoparticles in doses of 5 and 50 mg/kg caused no genotoxic effects in the same cells after 3-h and in a dose of 5 mg/kg after 7-day exposure. Silicon crystal nanoparticles in a dose of 50 mg/kg caused death of 60-80% mice after exposure <24 h. Injected in a dose of 50 mg/kg on days 1, 7, and 14 of gestation, silicon crystal nanoparticles reduced body weight gain in pregnant rats and newborn rats at different stages of the experiment, but had no effect on other parameters of physical development of rat progeny and caused no teratogenic effects.
将水悬浮液中呈1 - 5微米颗粒状的2 - 5纳米硅晶体纳米颗粒以单剂量腹腔注射到妊娠第1、7和14天的雄性F(1)(CBA×C57Bl/6)小鼠或远交白化大鼠体内。剂量为5、25和50毫克/千克的硅晶体纳米颗粒在暴露24小时后对小鼠骨髓细胞无细胞遗传活性，在暴露7天和14天后，剂量为5和25毫克/千克时也无细胞遗传活性。剂量为5毫克/千克的硅纳米颗粒暴露24小时后显著增加了骨髓细胞中的DNA损伤（通过DNA彗星试验检测）。剂量为50毫克/千克时，在相同暴露时间后，它们显著增加了骨髓和脑细胞中的DNA损伤。剂量为5和50毫克/千克的硅纳米颗粒在暴露3小时后对相同细胞无遗传毒性作用，剂量为5毫克/千克暴露7天后也无遗传毒性作用。剂量为50毫克/千克的硅晶体纳米颗粒在暴露<24小时后导致60 - 80%的小鼠死亡。在妊娠第1、7和14天以50毫克/千克的剂量注射硅晶体纳米颗粒，在实验的不同阶段降低了孕鼠和新生大鼠的体重增加，但对大鼠后代身体发育的其他参数没有影响，也未产生致畸作用。
