用于L-苯丙氨酸及其衍生物基因掺入的吡咯赖氨酸-tRNA合成酶的从头工程。
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives.
作者信息
Wang Yane-Shih, Russell William K, Wang Zhiyong, Wan Wei, Dodd Lindsey E, Pai Pei-Jing, Russell David H, Liu Wenshe R
机构信息
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
出版信息
Mol Biosyst. 2011 Mar;7(3):714-7. doi: 10.1039/c0mb00217h. Epub 2011 Jan 14.
PMID:21234492
Abstract
Using evolved pyrrolysyl-tRNA synthetase-tRNA(CUA)(Pyl) pairs, L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.
摘要
利用进化的吡咯赖氨酸-tRNA合成酶-tRNA(CUA)(Pyl)对，L-苯丙氨酸、对碘-L-苯丙氨酸和对溴-L-苯丙氨酸已被基因编码到大肠杆菌琥珀突变位点的蛋白质中。
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