Cardiovascular Research Center, School of Medicine and Pharmacology, University of Western Australia, Royal Perth Hospital, Western Australia, Australia.
Free Radic Res. 2011 Apr;45(4):469-76. doi: 10.3109/10715762.2010.544730. Epub 2011 Jan 14.
15-F₂(t)-isoprostane (15-F₂(t)-IsoP), an oxidation product of arachidonic acid (AA), affects vascular and platelet function; however, the bioactivity of other fatty acids oxidation products is unknown. This paper studied rat aortic vascular reactivity and human platelet aggregation in response to 14 oxidation products of AA, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and α-linolenic acid (ALA) compared with 15-F₂(t)-IsoP. It also compared the F₂(t)-IsoPs profile in human platelets. EPA-derived 15-F₃(t)-IsoP constricted rat aorta less than 15-F₂(t)-IsoP, but none of the other oxidation products affected vascular reactivity. Only 15-F₂(t)-IsoP (10⁻⁴ M) directly affected platelet aggregation. 15-F₃(t)-IsoP, ent-16-F₁-phytoprostane (from ALA) and isofurans A and B (from AA) inhibited reversible aggregation to U46619. Unlike plasma, the platelet profile of F₂-IsoP showed that 8-F(2t)-IsoP were higher than 15-F₂(t)-IsoP. Unlike 15-F₂(t)-IsoP, the test compounds derived from fatty acids oxidation did not affect vascular or platelet function. Elevated platelet 8-F₂(t)-IsoP could limit 15-F₂(t)-IsoP-induced aggregation under conditions of oxidant stress.
15-F₂(t)-异前列腺素（15-F₂(t)-IsoP）是花生四烯酸（AA）的氧化产物，影响血管和血小板功能；然而，其他脂肪酸氧化产物的生物活性尚不清楚。本文研究了与 15-F₂(t)-IsoP 相比，14 种 AA、二十碳五烯酸（EPA）、二十二碳六烯酸（DHA）和α-亚麻酸（ALA）氧化产物对大鼠主动脉血管反应性和人血小板聚集的影响。还比较了人血小板中 F₂(t)-IsoP 谱。与 15-F₂(t)-IsoP 相比，EPA 衍生的 15-F₃(t)-IsoP 收缩大鼠主动脉的作用较小，但其他氧化产物均不影响血管反应性。只有 15-F₂(t)-IsoP（10⁻⁴ M）直接影响血小板聚集。15-F₃(t)-IsoP、ent-16-F₁-植烷前列腺素（来自 ALA）和异呋烷 A 和 B（来自 AA）抑制 U46619 诱导的可逆聚集。与血浆不同，血小板 F₂-IsoP 谱显示 8-F(2t)-IsoP 高于 15-F₂(t)-IsoP。与 15-F₂(t)-IsoP 不同，这些源自脂肪酸氧化的测试化合物不影响血管或血小板功能。在氧化应激条件下，血小板 8-F₂(t)-IsoP 的升高可能限制 15-F₂(t)-IsoP 诱导的聚集。
