University of Western Australia, Perth, WA, Australia.
Free Radic Res. 2010 Sep;44(9):983-90. doi: 10.3109/10715762.2010.492830.
Omega-3 (omega3) fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), protect against cardiovascular disease. Despite these benefits, concern remains that omega3 fatty acids may increase lipid peroxidation. It has previously been shown that urinary F(2)-isoprostanes (F(2)-IsoPs) were reduced following omega3 fatty acid supplementation in humans. It is now determined whether EPA or DHA supplementation affects plasma F(2)-IsoPs. In two 6-week placebo-controlled interventions, Study A: overweight, dyslipidaemic men; and Study B: treated-hypertensive Type 2 diabetic, patients were randomized to 4 g daily EPA, DHA. Post-intervention plasma F(2)-IsoPs were significantly reduced by EPA (24% in Study A, 19% in Study B) and by DHA (14% in Study A, 23% in Study B) relative to the olive oil group. The fall in plasma F(2)-IsoPs was not altered in analyses that corrected for changes in plasma arachidonic acid, which was reduced with EPA and DHA supplementation. Neither F(3)- nor F(4)-IsoPs were observed in plasma in both studies. These results show that in humans, EPA and DHA reduce in vivo oxidant stress as measured in human plasma and urine.
ω-3（ω3）脂肪酸，特别是二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），可预防心血管疾病。尽管有这些益处，但人们仍然担心ω3 脂肪酸可能会增加脂质过氧化。先前已经表明，在人类补充 ω3 脂肪酸后，尿中 F（2）-异前列腺素（F（2）-IsoPs）减少。现在确定 EPA 或 DHA 补充是否会影响血浆 F（2）-IsoPs。在两项为期 6 周的安慰剂对照干预研究中，研究 A：超重、血脂异常的男性；和研究 B：治疗中的 2 型糖尿病高血压患者，随机分为每日 4 克 EPA、DHA。与橄榄油组相比，EPA（研究 A 中降低 24%，研究 B 中降低 19%）和 DHA（研究 A 中降低 14%，研究 B 中降低 23%）可显著降低干预后血浆 F（2）-IsoPs。在分析中，当校正血浆花生四烯酸的变化时，血浆 F（2）-IsoPs 的下降并没有改变，EPA 和 DHA 补充会降低血浆花生四烯酸。在这两项研究中，在血浆中均未观察到 F（3）-或 F（4）-IsoPs。这些结果表明，在人类中，EPA 和 DHA 可减少体内氧化应激，如通过测量人血浆和尿液中的 F（2）-IsoPs 来衡量。
