Wallbank A M, Drulak M, Poffenroth L, Barnes C, Kay C, Lebtag I
Health Lab Sci. 1978 Jul;15(3):133-7.
The National Institute of Health Guidelines for Recombinant DNA Research recommends 2% aqueous Wescodyne, an iodophore that is used in many hospitals and laboratories as a disinfectant, as a decontaminant for biological safety cabinets and 5% for a spill outside a cabinet. A contact time of 10 to 15 minutes was given for the 2% solution and 20 minutes was considered adequate for the 5% concentration. The results indicate: 1. Aqueous Wescodyne (5%) is ineffective when used for 80 minutes against poliovirus in a test mixture containing 8.5% bovine serum albumin (a mixture equivalent in protein concentration to the higher range in serum). 2. Wescodyne (10%) employed under the same conditions for 40 minutes is also ineffective. 3. Wescodyne (10% v/v) in 50% ethanol (w/w) was effective and this mixture, originally recommended for hand washing, should be considered for use in biohazard situations, particularly for decontamination of work surfaces and biological safety cabinets. These results are of significance for if a virucide cannot inactivate poliovirus one would be concerned about using the virucide against hepatitis B or SV40 viruses.
美国国立卫生研究院的重组DNA研究指南推荐使用2%的水性洗必泰碘（一种在许多医院和实验室用作消毒剂的碘伏）对生物安全柜进行去污处理，对于柜外溢出物则使用5%的浓度。2%溶液的接触时间为10至15分钟，5%浓度的接触时间20分钟被认为足够。结果表明：1. 在含有8.5%牛血清白蛋白（蛋白质浓度与血清较高范围相当的混合物）的测试混合物中，5%的水性洗必泰碘作用80分钟对脊髓灰质炎病毒无效。2. 相同条件下使用10%的洗必泰碘40分钟也无效。3. 50%乙醇（重量/重量）中10%（体积/体积）的洗必泰碘有效，这种最初推荐用于洗手的混合物应考虑用于生物危害情况，特别是用于工作表面和生物安全柜的去污处理。这些结果具有重要意义，因为如果一种杀病毒剂不能使脊髓灰质炎病毒失活，人们就会担心使用该杀病毒剂对抗乙型肝炎或SV40病毒的效果。
