Drulak M, Wallbank A M, Lebtag I
J Hyg (Lond). 1979 Aug;83(1):111-9. doi: 10.1017/s0022172400025870.
f2 bacteriophage in the presence of fetal calf serum (at a final concentration of 10%) was exposed to six commonly used disinfectants for times of 10, 20 and 30 sec. At the end of exposure times skim milk neutralized the disinfectant activity and residual virus was assayed using the plaque technique. The 6 disinfectants considered were Javex, sodium hydroxide, ethanol, Wescodyne, One Stroke Ves-Phene and Sonacide. A 0.25% (w/v) solution of sodium hydroxide and 1/50 Javex (1200 parts/10(6) chlorine) were the most effective of the six disinfectants considered since 10(5) f2 bacteriophage were inactivated in 30 seconds in each instance. Since a 0.25% (w/v) solution of sodium hydroxide had a pH of 12.5 this made it too caustic to use as a disinfectant in many practical situations. It was concluded therefore that Javex at some dilution less than 1/50 (greater than 1200 parts/10(6) chlorine) was the most practical of the six disinfectants to use. Ethanol (95%, v/v) inactivated 10(3) f2 bacteriophage in 30 seconds while 1/20 Wescodyne and undiluted Sonacide inactivated 10(1)-virus particles. Ves-Phene at a dilution of 1/50 was a completely ineffective virucide during the 30 sec exposure. The resistance of f2 bacteriophage to inactivation by these six disinfectants was compared with that of echovirus 11 and coxsackievirus B5. In all instances except exposure to undiluted Sonacide, f2 was comparable in resistance to inactivation and in many cases had greater resistance.
将f2噬菌体（存在终浓度为10%的胎牛血清）暴露于六种常用消毒剂中10、20和30秒。暴露时间结束时，脱脂乳中和消毒剂活性，使用蚀斑技术检测残留病毒。所考虑的六种消毒剂分别是Javex、氢氧化钠、乙醇、卫可、单步酚和索纳赛德。0.25%（w/v）氢氧化钠溶液和1/50的Javex（1200份/10⁶氯）是所考虑的六种消毒剂中最有效的，因为在每种情况下，10⁵个f2噬菌体在30秒内被灭活。由于0.25%（w/v）氢氧化钠溶液的pH值为12.5，这使得它在许多实际情况下作为消毒剂使用时腐蚀性太强。因此得出结论，稀释度小于1/50（大于1200份/10⁶氯）的Javex是六种消毒剂中最实用的。乙醇（95%，v/v）在30秒内灭活10³个f2噬菌体，而1/20的卫可和未稀释的索纳赛德灭活10¹个病毒颗粒。稀释度为1/50的酚在30秒暴露期间是一种完全无效的杀病毒剂。将f2噬菌体对这六种消毒剂灭活的抗性与埃可病毒11型和柯萨奇病毒B5型的抗性进行了比较。除暴露于未稀释的索纳赛德外，在所有情况下，f2的灭活抗性相当，在许多情况下具有更强的抗性。
