Department of Traumatology and Orthopaedics, Charité - Universitätsmedizin, Berlin, Germany.
Int Orthop. 2011 Nov;35(11):1719-23. doi: 10.1007/s00264-010-1178-2. Epub 2011 Jan 16.
In septic joint surgery, the most frequently used antiseptics are polyhexanide, hydrogen peroxide and taurolidine. The aim of this study was to examine the effects of these antiseptics on viability of human chondrocytes. Our hypothesis was that antiseptics and supplemental irrigation with sodium chloride lavage are less toxic on human chondrocytes than treatment with antiseptics only. Primary human chondrocytes were isolated and cultured from six donated human knee joints. Polyhexanide, hydrogen peroxide or taurolidine were added to the cultures. Toxicity analysis was performed by visualisation of cell structure using light microscopy and LDH activity. The determination of vital cells and total cell numbers of chondrocytes treated with antiseptics partly followed by irrigation with sodium chloride solution was performed by using Casy Cell-Counter. Light microscopic data revealed a defect in cell structure after addition of antiseptics. We showed a significant increase of LDH enzyme activity after the treatment with polyhexanide or taurolidine. After treatment with antiseptics followed by sodium chloride solution a significant increase of vital and total cell numbers resulted in comparison with the chondrocytes that were only treated with antiseptics. The data show that treatment with polyhexanid, hydrogen peroxide or taurolidine induces cell death of human chondroctes in vitro. The application of sodium chloride solution after the treatment with polyhexanide and hydrogen peroxide possibly has a protective effect on chondrocyte viability.
在感染性关节手术中，最常使用的防腐剂是聚己双胍、过氧化氢和牛磺罗定。本研究旨在研究这些防腐剂对人软骨细胞活力的影响。我们的假设是，防腐剂和补充氯化钠灌洗对人软骨细胞的毒性小于单独使用防腐剂。从六个捐赠的人膝关节中分离和培养原代人软骨细胞。将聚己双胍、过氧化氢或牛磺罗定添加到培养物中。通过使用 Casy Cell-Counter 进行细胞活力分析和 LDH 活性分析来评估毒性。通过使用 Casy Cell-Counter 分析用防腐剂处理部分后用氯化钠溶液冲洗的软骨细胞的活细胞和总细胞数。光镜数据显示加入防腐剂后细胞结构出现缺陷。我们发现聚己双胍或牛磺罗定处理后 LDH 酶活性显著增加。用防腐剂处理后再用氯化钠溶液处理，与仅用防腐剂处理的软骨细胞相比，活细胞和总细胞数显著增加。数据表明，聚己双胍、过氧化氢或牛磺罗定处理在体外诱导人软骨细胞死亡。用聚己双胍和过氧化氢处理后应用氯化钠溶液可能对软骨细胞活力具有保护作用。
