牛磺罗定凝胶对离体牙周生物膜的作用。
Activity of taurolidine gels on ex vivo periodontal biofilm.
机构信息
School of Dental Medicine, Department of Periodontology, University of Bern, Freiburgstrasse 7, CH-3010, Bern, Switzerland.
出版信息
Clin Oral Investig. 2018 Jun;22(5):2031-2037. doi: 10.1007/s00784-017-2297-6. Epub 2017 Dec 14.
OBJECTIVES
The purpose of this study is to evaluate the activity of two different taurolidine (TAU) gels in comparison with a 0.2% chlorhexidine (CHX) gel on an ex vivo subgingival biofilm.
MATERIAL AND METHODS
Subgingival including supragingival biofilm samples from periodontitis patients were cultured for 10 days, before TAU 1% and TAU 3% gels and CHX gel were applied for 10 min and thereafter diluted with nutrient media to 10% for 50 min. One third of the samples were analyzed for bacterial counts, biofilm quantity, and biofilm metabolic activity. In the two other thirds, 90% of the nutrient media were replaced and biofilms were incubated for 23 h. The second third was analyzed in the same way as before. In the third part, patients' microorganisms were added again and incubated for additional 24 h to allow reformation of biofilm before proceeding to analysis.
RESULTS
Decrease of bacterial counts in biofilms was highest following application of TAU 3% after 60 min (0.87 log cfu, corresponding 86.5%), 24 and 48 h (reformation of biofilms), respectively. All antimicrobials reduced biofilm quantity after 24 h (each p < 0.05) and following reformation of biofilms (each p < 0.01). Metabolic activity in biofilms was decreased at 60 min (each p < 0.05) and at 24 h (each p < 0.01) after application of TAU gels, while the activity of the reformed biofilm was lower after application of all evaluated antimicrobials (each p < 0.01) than in the control group (e.g., without exposure to antimicrobials).
CONCLUSION
The antimicrobial activity of taurolidine gels clearly depends on its taurolidine concentration. A high concentrated taurolidine gel is equally active or even superior to 0.2% chlorhexidine gel. However, the activity of antimicrobials is limited in a complex established biofilm and underlines the pivotal role of mechanical biofilm disruption.
CLINICAL RELEVANCE
Within their limits, the data suggest that TAU 3% gel might represent a potential alternative to 0.2% chlorhexidine gel.
目的
本研究旨在评估两种不同的牛磺罗定（TAU）凝胶与 0.2%洗必泰（CHX）凝胶在体外龈下生物膜上的活性。
材料与方法
从牙周炎患者的龈下包括龈上生物膜样本中培养 10 天，然后将 TAU 1%和 TAU 3%凝胶和 CHX 凝胶应用 10 分钟，然后用营养培养基稀释至 10%50 分钟。三分之一的样本用于分析细菌计数、生物膜量和生物膜代谢活性。在另外三分之二的样本中，更换 90%的营养培养基，并孵育生物膜 23 小时。第二个三分之一以同样的方式进行分析。在第三部分中，再次添加患者的微生物并孵育 24 小时以允许生物膜重新形成，然后再进行分析。
结果
应用 TAU 3%后，生物膜中细菌计数的减少最高，60 分钟后为 0.87 log cfu（对应 86.5%），24 和 48 小时（生物膜重新形成），分别。所有抗菌剂在 24 小时后均减少生物膜量（p<0.05），并在生物膜重新形成后（p<0.01）。在应用 TAU 凝胶后，60 分钟（p<0.05）和 24 小时（p<0.01）时生物膜的代谢活性降低，而在应用所有评估的抗菌剂后，重新形成的生物膜的活性低于对照组（例如，不接触抗菌剂）（p<0.01）。
结论
牛磺罗定凝胶的抗菌活性明显取决于其牛磺罗定浓度。高浓度的牛磺罗定凝胶与 0.2%洗必泰凝胶同样有效，甚至更有效。然而，抗菌剂的活性在复杂的已建立的生物膜中受到限制，并强调了机械生物膜破坏的关键作用。
临床意义
在其限制范围内，数据表明 TAU 3%凝胶可能是 0.2%洗必泰凝胶的潜在替代品。
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