Department of Periodontology, School of Dentistry, Pusan National University, Yangsan City, South Korea.
J Periodontal Res. 2011 Apr;46(2):240-5. doi: 10.1111/j.1600-0765.2010.01339.x. Epub 2011 Jan 18.
Heat shock protein 60 (HSP60) of Porphyromonas gingivalis, a major periodontal pathogen, might be a trigger molecule linking infectious periodontitis and autoimmune atherosclerosis. The aim of this study was to identify the peptide specificity of anti-P. gingivalis HSP60 monoclonal antibodies and their cross-reactivity with bacterial and human HSPs. Their specific immunoreactivity to periodontal or atherosclerotic lesions was also investigated.
Twenty patients with chronic periodontitis and 20 atherosclerosis patients who had undergone surgical intervention for atheromatous plaques with evidence of ongoing periodontal disease, were selected. Synthetic peptide 19 ((TLVVNRLRGSLKICAVKAPG)-specific T-cell lines were established from inflamed gingiva and atheromatous plaque and the phenotypes and cytokine profiles were characterized.
Thirty per cent of periodontitis patients and 100% of atherosclerosis patients reacted positively to cross-reactive peptide 19 from both P. gingivalis and human HSP60. The peptide 19-specific T-cell lines demonstrated the phenotype characteristic of helper T cells (CD4(+)) but did not express CD25 or FOXP3. The interleukin-10 levels were elevated significantly in the peptide 19 T-cell line.
Synthetic peptide 19 of P. gingivalis HSP60 is an immunoreactive epitope in the periodontitis-atherosclerosis axis.
牙龈卟啉单胞菌（一种主要的牙周病原体）的热休克蛋白 60（HSP60）可能是连接感染性牙周炎和自身免疫性动脉粥样硬化的触发分子。本研究旨在确定抗牙龈卟啉单胞菌 HSP60 单克隆抗体的肽特异性及其与细菌和人 HSP 的交叉反应性，并研究其对牙周或动脉粥样硬化病变的特异性免疫反应。
选择 20 例慢性牙周炎患者和 20 例因动脉粥样硬化斑块而行手术干预的患者，这些患者有证据表明存在进行性牙周病。从炎症性牙龈和动脉粥样硬化斑块中建立了针对合成肽 19（（TLVVNRLRGSLKICAVKAPG））的特异性 T 细胞系，并对其表型和细胞因子谱进行了特征描述。
30%的牙周炎患者和 100%的动脉粥样硬化患者对来自牙龈卟啉单胞菌和人 HSP60 的交叉反应性肽 19 呈阳性反应。肽 19 特异性 T 细胞系表现出辅助性 T 细胞（CD4+）的表型特征，但不表达 CD25 或 FOXP3。IL-10 水平在肽 19 T 细胞系中显著升高。
牙龈卟啉单胞菌 HSP60 的合成肽 19 是牙周炎-动脉粥样硬化轴中的一个免疫反应性表位。
