Department of Immunology, Collegium Medicum Nicolaus Copernicus University, Bydgoszcz, Poland.
Helicobacter. 2011 Feb;16(1):27-35. doi: 10.1111/j.1523-5378.2010.00809.x.
In this study, H. pylori-infected and noninfected children with gastritis were compared to a control group with respect to circulating CD4(+) and CD8(+) T lymphocytes expressing activation and differentiation markers. Additionally, the lymphocyte phenotypes of children with gastritis were correlated with the gastric inflammation scores.
H. pylori infection status was assessed based on [¹³C]urea breath test, rapid urease test, and histology. Analysis of the lymphocyte surface molecule expression was carried out by triple-color flow cytometry.
The group of H. pylori-infected children showed an elevated proportion of peripheral B cells with CD19(low) , along with a twofold increase in the percentage of memory (CD45RO(+)) CD4(+) and CD8(+) T-cell subsets (p < .05). Moreover, a positive correlation between the age and the percentage of these subsets was seen (r = .38, p = .04 and r = .56, p < .01, respectively). Children with gastritis but without infection had a slightly increased percentage of CD8(+) T cells and CD56(+) NK cells, CD3(high) T cells and CD45RO(high) CD4(+) T-cell subsets (p < .05). Both H. pylori-infected and noninfected children with gastritis were characterized by an increased percentage of memory/effector CD4(+) T cells, the presence of NK cells with CD56(high), memory T-cell subset with CD4(high), and naive, memory, memory/effector, and effector T-cell subsets with CD8(high) (p < .05). Gastric inflammation scores correlated positively with the percentage of CD4(+) T lymphocytes in H. pylori-infected children (r = .42, p = .03). In noninfected children, gastric inflammation scores correlated positively with the percentage of B cells (r = .45, p = .04).
In H. pylori-negative children, gastritis was associated with an increased percentage of activated NK and T cells, and intermediate-differentiated peripheral blood CD4(+) T cells, which was more pronounced in H. pylori-positive children who also showed an increased B-cell response. However, increased inflammation was only associated with the elevation of CD4(+) T-cell percentage in H. pylori-positive children as well as B-cell percentage in H. pylori-negative children with gastritis.
本研究比较了 H. pylori 感染和非感染性胃炎患儿与对照组之间循环 CD4(+)和 CD8(+)T 淋巴细胞的活化和分化标志物的表达。此外，还将胃炎患儿的淋巴细胞表型与胃炎症评分相关联。
基于 [¹³C]尿素呼气试验、快速尿素酶试验和组织学检查评估 H. pylori 感染状态。采用三色流式细胞术分析淋巴细胞表面分子表达。
H. pylori 感染组患儿外周血 B 细胞 CD19(low)比例升高，记忆性（CD45RO(+)) CD4(+)和 CD8(+)T 细胞亚群比例增加两倍（p<.05）。此外，还观察到年龄与这些亚群比例之间存在正相关（r =.38，p =.04 和 r =.56，p <.01）。无 H. pylori 感染的胃炎患儿 CD8(+)T 细胞和 CD56(+)NK 细胞、CD3(high)T 细胞和 CD45RO(high)CD4(+)T 细胞亚群比例略有增加（p<.05）。H. pylori 感染和非感染性胃炎患儿均表现为记忆/效应 CD4(+)T 细胞比例增加，存在 CD56(high)NK 细胞、CD4(high)记忆 T 细胞亚群以及幼稚、记忆、记忆/效应和效应 CD8(high)T 细胞亚群（p<.05）。胃炎症评分与 H. pylori 感染患儿 CD4(+)T 淋巴细胞比例呈正相关（r =.42，p =.03）。在非感染患儿中，胃炎症评分与 B 细胞比例呈正相关（r =.45，p =.04）。
在 H. pylori 阴性儿童中，胃炎与活化 NK 和 T 细胞以及外周血 CD4(+)T 细胞的中间分化增加有关，在 H. pylori 阳性儿童中更为明显，这些儿童还表现出 B 细胞反应增加。然而，仅在 H. pylori 阳性儿童中，炎症增加与 CD4(+)T 细胞比例升高以及 H. pylori 阴性胃炎患儿的 B 细胞比例升高相关。
