Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.
Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland ; Department of Regenerative Medicine and Immune Regulation, Medical University of Białystok, Waszyngtona 13 Street, 15-269 Białystok, Poland.
J Immunol Res. 2015;2015:106743. doi: 10.1155/2015/106743. Epub 2015 Feb 22.
The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.
本研究旨在探讨特定的乳酸菌（LAB）菌株（体外对幽门螺杆菌具有拮抗或非拮抗作用）在调节树突状细胞（DC）成熟表型和细胞因子表达模式方面的能力是否存在差异。方法：通过直接暴露于具有拮抗（L. rhamnosus 900）和非拮抗（L. paracasei 915）作用的 LAB 菌株或含有 cagA+的幽门螺杆菌菌株，来诱导单核细胞来源的 DC 成熟。根据表面标志物表达和细胞因子产生来评估 DC 成熟特征。结果：我们观察到 LAB 菌株和 LAB 与幽门螺杆菌的混合物均能够诱导成熟的 DC。与此同时，与 L. rhamnosus 900 相比，L. paracasei 915 导致高水平的 IL-10/IL-12p70 细胞因子比值。结论：本研究表明，所分析的乳酸菌菌株比幽门螺杆菌更能刺激 DC 成熟。有趣的是，在所选择的两种 LAB 菌株中，对幽门螺杆菌具有拮抗作用的 L. rhamnosus 900 具有更强的促炎和可能的杀菌特性。
