Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970, Taiwan.
Genomics. 2011 Apr;97(4):249-55. doi: 10.1016/j.ygeno.2011.01.002. Epub 2011 Jan 15.
We present the complete genomic sequence of a lytic bacteriophage ϕAB1 which can infect many clinical isolates of multidrug-resistant Acinetobacter baumannii. The recently isolated bacteriophage displays morphology resembling Podoviridae family. The ϕAB1 genome is a linear double-stranded DNA of 41,526 bp containing 46 possible open reading frames (ORFs). The majority of the predicted structural proteins were identified as part of the phage particle by mass spectrometry analysis. According to the virion morphology, overall genomic structure, and the phylogenetic tree of RNA polymerase, we propose that ϕAB1 is a new member of the ϕKMV-like phages. Additionally, we identified four ORFs encoding putative HNH endonucleases, one of which is presumed to integrate and create a genes-in-pieces DNA polymerase. Also, a potential lysis cassette was identified in the late genome. The lytic power of this bacteriophage combined with its specificity for A. baumannii makes ϕAB1 an attractive agent for therapeutic or disinfection applications.
我们呈现了一种能够感染许多多药耐药鲍曼不动杆菌临床分离株的裂解噬菌体 ϕAB1 的完整基因组序列。最近分离的噬菌体显示出类似于 Podoviridae 科的形态。ϕAB1 基因组是一条长 41,526 bp 的线性双链 DNA，包含 46 个可能的开放阅读框（ORFs）。通过质谱分析，大多数预测的结构蛋白被鉴定为噬菌体颗粒的一部分。根据病毒粒子形态、整体基因组结构和 RNA 聚合酶的系统发育树，我们提出 ϕAB1 是 ϕKMV 样噬菌体的一个新成员。此外，我们鉴定了四个编码假定 HNH 内切酶的 ORF，其中一个被认为是整合并创建基因片段 DNA 聚合酶的酶。此外，在晚期基因组中鉴定出一个潜在的裂解盒。这种噬菌体的裂解能力及其对鲍曼不动杆菌的特异性使 ϕAB1 成为治疗或消毒应用的有吸引力的制剂。
