Liu Shijuan, Liu Changju, Wang Xue, Chen Huiqing
School of Life Science, Qufu Normal University, Qufu, 273165, China.
Plant Cell Rep. 2021 Jan;40(1):213-221. doi: 10.1007/s00299-020-02627-8. Epub 2020 Oct 24.
The promoter of the Arabidopsis thaliana β-glucosidase 19 gene directs GUS expression in a seed-specific manner in transgenic Arabidopsis and tobacco. In the present study, an 898-bp putative promoter of the Arabidopsis β-glucosidase 19 (AtBGLU19) gene was cloned. The bioinformatics analysis of the cis-acting elements indicated that this putative promoter contains many seed-specific elements, such as RY elements. The features of this promoter fragment were evaluated for the capacity to direct the β-glucuronidase (GUS) reporter gene in transgenic Arabidopsis and tobacco. Histochemical and fluorometric GUS analyses of transgenic Arabidopsis plants revealed that the AtBGLU19 promoter directed strong GUS activity in late-maturing seeds and dry seeds, whereas no GUS expression was observed in other organs. The results indicated that the AtBGLU19 promoter was able to direct GUS expression in a seed-specific manner in transgenic Arabidopsis. In tobacco, the intensity of the staining and the level of GUS activity were considerably higher in the seeds than in the other tissues. These results further confirmed that the AtBGLU19 promoter is seed specific and can be used to control transgene expression in a heterologous plant system.
拟南芥β-葡萄糖苷酶19基因的启动子在转基因拟南芥和烟草中以种子特异性方式指导GUS表达。在本研究中，克隆了拟南芥β-葡萄糖苷酶19（AtBGLU19）基因一个898bp的推定启动子。对顺式作用元件的生物信息学分析表明，该推定启动子含有许多种子特异性元件，如RY元件。对该启动子片段的特性进行了评估，以确定其在转基因拟南芥和烟草中指导β-葡萄糖醛酸酶（GUS）报告基因的能力。对转基因拟南芥植株进行的组织化学和荧光GUS分析表明，AtBGLU19启动子在成熟后期种子和干燥种子中指导强烈的GUS活性，而在其他器官中未观察到GUS表达。结果表明，AtBGLU19启动子能够在转基因拟南芥中以种子特异性方式指导GUS表达。在烟草中，种子中的染色强度和GUS活性水平明显高于其他组织。这些结果进一步证实，AtBGLU19启动子具有种子特异性，可用于在异源植物系统中控制转基因表达。
