氧合酶 CmmOIV 催化灰霉素生物合成途径中末端激活步骤的特性。
Characterization of the terminal activation step catalyzed by oxygenase CmmOIV of the chromomycin biosynthetic pathway from Streptomyces griseus.
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536-0596, USA.
出版信息
Biochemistry. 2011 Mar 1;50(8):1421-8. doi: 10.1021/bi1016205. Epub 2011 Feb 2.
Abstract
Inactivation and initial interrogation of key oxygenase CmmOIV of the biosynthetic pathway of chromomycin A(3) in Streptomyces griseus ssp. griseus revealed that a completely methylated and acetylated prechromomycin is the preferred substrate of this enzyme. This suggests that the three sugar decoration reactions, two O-acetylations and an O-methylation, which were previously believed to occur as the final steps of chromomycin A(3) biosynthesis, indeed take place prior to the CmmOIV reaction. Upon inactivation of CmmOIV, four new compounds accumulated; the fully decorated prechromomycin and its incompletely acetylated precursor along with a diketoprechromomycin-type compound were fully characterized and assayed with CmmOIV.
摘要
在灰色链霉菌灰色亚种生物合成途径中关键加氧酶 CmmOIV 的失活和初步检测表明，完全甲基化和乙酰化的前色霉素是该酶的首选底物。这表明，先前被认为是色霉素 A(3)生物合成最后步骤的三个糖修饰反应，即两个 O-乙酰化和一个 O-甲基化，实际上确实发生在 CmmOIV 反应之前。在 CmmOIV 失活后，积累了四种新化合物；完全修饰的前色霉素及其不完全乙酰化的前体以及二酮前色霉素型化合物都得到了充分的表征，并与 CmmOIV 进行了检测。
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