Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000 Gent, Belgium.
Environ Microbiol. 2011 Apr;13(4):1042-51. doi: 10.1111/j.1462-2920.2010.02410.x. Epub 2011 Jan 18.
Poly-β-hydroxybutyrate (PHB) is a bacterial energy and carbon storage compound which exhibits a controlling effect on the gastrointestinal microbiota. Its beneficial activities for aquaculture have already been shown in terms of increased disease resistance and growth performance in a number of studies. However, the action of PHB on the intestinal microbial community in the treated animals has not yet been studied in depth. In this research, the effects of PHB on the microbiota composition in the intestinal tract of juvenile sea bass were examined. It was found that fish cohabiting in the same tank were on average 87% similar regarding the intestinal microbiota. When subjected to the same treatment and environmental conditions but reared in different tanks, the compositions of the enteric communities diverged. The provision of PHB overruled this tank effect by sustaining a microbial core community in the gut that represented 60% of the total bacterial diversity at the highest PHB level of 10%. The microbial community compositions converged between replicate tanks upon supplementation of PHB and were characterized by high dynamics and increased evenness. The results are discussed in the framework of hypotheses that try to relate the intestinal microbial community composition to the health status of the host organisms.
聚-β-羟基丁酸（PHB）是一种细菌能量和碳储存化合物，对胃肠道微生物群具有控制作用。在许多研究中，已经证明其对水产养殖具有有益的作用，可提高疾病抵抗力和生长性能。然而，PHB 对处理动物肠道微生物群落的作用尚未得到深入研究。在这项研究中，研究了 PHB 对幼鲈肠道微生物群落组成的影响。结果发现，同一水箱中共同养殖的鱼类肠道微生物的平均相似度为 87%。当它们处于相同的处理和环境条件下，但在不同的水箱中养殖时，肠道群落的组成则存在差异。提供 PHB 可通过维持肠道中的微生物核心群落来克服这种水箱效应，在 PHB 水平最高为 10%时，该核心群落代表了总细菌多样性的 60%。补充 PHB 后，重复水箱之间的微生物群落组成趋于一致，其特征是动态性高且均匀度增加。这些结果是在试图将肠道微生物群落组成与宿主生物体健康状况相关联的假设框架内进行讨论的。
