Mechanobiology Institute, National University of Singapore, Singapore.
Biophys J. 2011 Jan 19;100(2):517-23. doi: 10.1016/j.bpj.2010.12.3700.
Although magnetic tweezers have many unique advantages in terms of specificity, throughput, and force stability, this tool has had limited application on short tethers because accurate measurement of force has been difficult for short tethers under large tension. Here, we report a method that allows us to apply magnetic tweezers to stretch short biomolecules with accurate force calibration over a wide range of up to 100 pN. We demonstrate the use of the method by overstretching of a short DNA and unfolding/refolding a protein of filamin A immunoglobulin domains 1-8. Other potential applications of this method are also discussed.
尽管磁镊在特异性、通量和力稳定性方面具有许多独特的优势，但由于在大张力下难以对短系绳进行精确的力测量，因此该工具在短系绳上的应用受到限制。在这里，我们报告了一种方法，该方法允许我们在高达 100 pN 的广泛范围内对短生物分子进行精确的力校准和拉伸。我们通过过度拉伸短 DNA 和展开/折叠纤连蛋白免疫球蛋白结构域 1-8 的蛋白质来演示该方法的用途。还讨论了该方法的其他潜在应用。
