ETH Zurich, Switzerland.
Brief Bioinform. 2011 Sep;12(5):489-97. doi: 10.1093/bib/bbq077. Epub 2011 Jan 18.
Next-generation sequencing platforms are dramatically reducing the cost of DNA sequencing. With these technologies, bases are inferred from light intensity signals, a process commonly referred to as base-calling. Thus, understanding and improving the quality of sequence data generated using these approaches are of high interest. Recently, a number of papers have characterized the biases associated with base-calling and proposed methodological improvements. In this review, we summarize recent development of base-calling approaches for the Illumina and Roche 454 sequencing platforms.
下一代测序平台大大降低了 DNA 测序的成本。在这些技术中，碱基是从光强信号推断出来的，这一过程通常被称为碱基调用。因此，理解和提高使用这些方法生成的序列数据的质量是非常重要的。最近，有许多论文描述了与碱基调用相关的偏倚，并提出了方法上的改进。在这篇综述中，我们总结了 Illumina 和 Roche 454 测序平台的碱基调用方法的最新进展。
