Neurosurgery Research Group, CHUV-UNIL, CH-1011 Lausanne, Switzerland.
J Comp Neurol. 2011 Mar 1;519(4):775-89. doi: 10.1002/cne.22547.
We have demonstrated that cortical cell autografts might be a useful therapy in two monkey models of neurological disease: motor cortex lesion and Parkinson's disease. However, the origin of the useful transplanted cells obtained from cortical biopsies is not clear. In this report we describe the expression of doublecortin (DCX) in these cells based on reverse-transcription polymerase chain reaction (RT-PCR) and immunodetection in the adult primate cortex and cell cultures. The results showed that DCX-positive cells were present in the whole primate cerebral cortex and also expressed glial and/or neuronal markers such as glial fibrillary protein (GFAP) or neuronal nuclei (NeuN). We also demonstrated that only DCX/GFAP positive cells were able to proliferate and originate progenitor cells in vitro. We hypothesize that these DCX-positive cells in vivo have a role in cortical plasticity and brain reaction to injury. Moreover, in vitro these DCX-positive cells have the potential to reacquire progenitor characteristics that confirm their potential for brain repair.
我们已经证明，皮质细胞自体移植可能是两种神经疾病猴模型（运动皮层损伤和帕金森病）的一种有效治疗方法。然而，从皮质活检中获得的有用移植细胞的起源尚不清楚。在本报告中，我们通过逆转录聚合酶链反应（RT-PCR）和免疫检测，描述了成年灵长类皮质和细胞培养物中这些细胞的双皮质素（DCX）表达。结果表明，DCX 阳性细胞存在于整个灵长类大脑皮质中，并且还表达神经胶质和/或神经元标志物，如神经胶质原纤维酸性蛋白（GFAP）或神经元核（NeuN）。我们还证明，只有 DCX/GFAP 阳性细胞能够在体外增殖并产生祖细胞。我们假设，这些体内的 DCX 阳性细胞在皮质可塑性和大脑对损伤的反应中具有作用。此外，在体外，这些 DCX 阳性细胞具有重新获得祖细胞特征的潜力，证实了它们对大脑修复的潜力。
